PMID: 9174167May 1, 1997Paper

Molecular cloning and gene expression analysis of PSP94 (prostate secretory protein of 94 amino acids) in primates

DNA and Cell Biology
J W XuanJ L Chin

Abstract

Prostate secretory protein of 94 amino acids (PSP94) has shown the potential to be a diagnostic biomarker and a therapeutic agent for prostate cancer. Primates have been the main animal models for studying the biology of this molecule. We have cloned and analyzed the cDNA and promoter region of PSP94 from baboon (Papio anubis). Sequence divergence among baboon, monkey, pig, and human, in both the exons and 5'-flanking region indicates rapid evolution of the PSP94 gene. There are conserved steroid hormone response elements (SHRE) in the promoter region of all three primate species. Multiple, alternative transcripts starting near these SHREs and upstream to the TATA box were identified by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of 5'-cDNA ends (5' RACE) in primate prostatic tissues. This differential transcription initiation may be linked to androgen regulation of PSP94 gene expression. PSP94 transcripts were detected by RT-PCR in a wide variety of mucus-secreting tissues. However, the alternative transcripts were found only in the prostate. The distribution of the PSP94 protein in baboon secretory tissues was also examined by Western blot analysis using a polyclonal antibody against the h...Continue Reading

References

Jan 1, 1991·Urological Research·H von der KammerT G Cooper
Oct 15, 1991·Biochemical and Biophysical Research Communications·Z G LiangS S Koide
Jan 1, 1990·Methods in Enzymology·T GojoboriM Kimura
Mar 30, 1990·Biochemical and Biophysical Research Communications·C B GreenS C Kwok
Feb 10, 1989·Cell·M Beato
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P ShawC K Shen
May 1, 1987·Biology of Reproduction·W L GordonD N Ward
Jun 10, 1985·Biochimica Et Biophysica Acta·K AkiyamaM Hara
Feb 15, 1994·Archives of Biochemistry and Biophysics·P FernlundP Roepstorff

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Citations

Sep 22, 1999·European Journal of Biochemistry·M MäkinenA Lundwall
Aug 23, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Nariaki FujimotoJun Kanno
Jun 18, 2010·Biochimica Et Biophysica Acta·Dhanashree D JagtapSmita D Mahale
Jan 29, 2008·Biochimica Et Biophysica Acta·Narumi AokiShigeyuki Terada
Feb 11, 2005·Journal of Molecular Biology·Iren WangChinpan Chen
Aug 26, 2006·Journal of Molecular Biology·Houman GhasrianiTorbjörn Drakenberg
May 13, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·N Anahí FranchiCarlos E Coronel
Jul 1, 2006·Cancer Investigation·Peter A CadieuxMichael W Clarke

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