Molecular Cloning and Identification of the 2'-5' Oligoadenylate Synthetase 2 Gene in Chinese Domestic Pigs Through Bioinformatics Analysis, and Determination of Its Antiviral Activity Against Porcine Reproductive and Respiratory Syndrome Virus Infection

Indian Journal of Microbiology
Ruining WangMengmeng Zhao

Abstract

An interferon-mediated antiviral protein, 2'-5' oligoadenylate synthetase 2, plays an important role in the antiviral response of interferons. In this study, 2'-5' oligoadenylate synthetase 2 genes were cloned from Chinese domestic pigs. Bioinformatics analysis revealed that the 2024-bp long open reading fame encodes 707 amino acids. There are two conserved regions in this protein: the nucleotidyltransferase domain, and the 2'-5' oligoadenylate synthetase domain (OAS). Genetic evolution analysis showed that the 2'-5' oligoadenylate synthetase 2 gene in domestic pigs is closely related to that of cattle. There are multiple antigenic sites, no signal peptide, and no transmembrane region in the gene, which is predicted to be a hydrophilic protein. Secondary structures were found to be mainly alpha helix-based; its tertiary structure is close to that of humans and cattle, but not that of mice. Tissue distribution results indicated that this protein is distributed in multiple organs, with high distribution in the liver; it is mainly localized in the cytoplasm. PRRSV infection, interferon-beta, and Poly(I: C) treatment all promoted 2'-5' oligoadenylate synthetase 2 gene expression. Overexpression and RNA silencing of porcine OAS2 inh...Continue Reading

References

Feb 1, 1979·European Journal of Biochemistry·A G Hovanessian, I M Kerr
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D A BenfieldD Chladek
Aug 29, 1997·The Journal of Biological Chemistry·B Dong, R H Silverman
Apr 14, 1999·Cell Death and Differentiation·J C CastelliR J Youle
Apr 13, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Y YamamotoY Sokawa
Mar 16, 2005·Biochemical and Biophysical Research Communications·Emi HamanoNaoto Keicho
Jan 23, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·K AlagarasuD Cecilia
Aug 2, 2015·Current Opinion in Virology·Melissa Drappier, Thomas Michiels
Nov 20, 2015·Emerging Infectious Diseases·Lei ZhouHanchun Yang
Aug 2, 2017·International Journal of Molecular Sciences·Mengmeng ZhaoShitao Li

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Methods Mentioned

BETA
PCR
Assay
glycosylation
Infection
transfection

Software Mentioned

SignalP
ProtSCale
PSORT
NetNGlyc
MEGA
mode
ClustalX
NetPhos
Protparam
Swiss

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