Molecular cloning and immune responsive expression of MDA5 gene, a pivotal member of the RLR gene family from grass carp Ctenopharyngodon idella

Fish & Shellfish Immunology
Jianguo SuLimin Peng

Abstract

The cytoplasmic helicase protein MDA5 (melanoma-differentiation-associated gene-5) recognizes long molecules of viral double-stranded RNA (dsRNA) and single-stranded RNA with 5' triphosphate and mediates type I interferon secretion. In the present study, the first MDA5 gene in fish was cloned and characterized from grass carp Ctenopharyngodon idella. The full length of the C. idella MDA5 (CiMDA5) cDNA is 3233 nucleotides in length and encodes a polypeptide of 961 amino acids. The deduced amino acid sequence contained six main structural domains: a CARD (caspase activation and recruitment domain), a DEXDc (DEAD/DEAH box helicase domain), a ResIII (conserved restriction domain of bacterial type III restriction enzyme), two HELICc (helicase superfamily c-terminal domain) and an RD (regulatory domain). The CiMDA5 mRNA was widespread expression in the tested tissues, was high level in spleen, skin and gill tissues, and was up-regulated by GCRV injection by semi-quantitative RT-PCR assay. The CiMDA5 transcripts in spleen were significantly up-regulated at 12 h (1.80 folds, P < 0.05), reached the crest at 24 h (7.48 folds, P < 0.05), and then recovered to normal level at 48 h post-injection (P > 0.05) of grass carp reovirus (GCRV). In...Continue Reading

References

Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dong-chul KangPaul B Fisher
May 12, 2004·Current Drug Targets·Jason S Damiano, John C Reed
Feb 11, 2005·Current Opinion in Gastroenterology·Zhiping Li, Anna Mae Diehl
Aug 30, 2005·Nature Immunology·Taro KawaiShizuo Akira
Mar 31, 2007·The Journal of Biological Chemistry·Mitsutoshi Yoneyama, Takashi Fujita
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Feb 15, 2008·Current Opinion in Immunology·Osamu Takeuchi, Shizuo Akira
Oct 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Devanand SarkarPaul B Fisher
Feb 28, 2009·International Immunology·Taro Kawai, Shizuo Akira
Apr 30, 2009·BMC Evolutionary Biology·Jun ZouChris J Secombes
Aug 7, 2009·Journal of Virology·Andreas PichlmairCaetano Reis e Sousa

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Citations

Jun 5, 2013·Developmental and Comparative Immunology·Chunrong YangLimin Peng
Mar 7, 2013·Fish & Shellfish Immunology·Takashi AokiTae Sung Jung
Dec 4, 2012·International Journal of Molecular Sciences·Lei GaoWeidong Liu
May 17, 2014·SpringerPlus·Xiu-Ying YanZao-He Wu
Jun 19, 2012·Annual Review of Genomics and Human Genetics·Thomas BoehmIsabell Hess
Jul 18, 2015·Biology·Sarah PoynterStephanie DeWitte-Orr
Oct 1, 2013·Journal of Molecular Biology·Christelle LangevinPierre Boudinot
Apr 17, 2012·Developmental and Comparative Immunology·Lv-yun ZhuJian-zhong Shao
Mar 31, 2012·Comparative Immunology, Microbiology and Infectious Diseases·Chih-Chun LeeTsang Long Lin
Jul 26, 2011·Developmental and Comparative Immunology·Jun Zou, Christopher J Secombes
Jan 19, 2011·Developmental and Comparative Immunology·John D HansenKerry J Laing
Dec 28, 2010·Developmental and Comparative Immunology·Maki OhtaniTakashi Aoki
Apr 7, 2016·Fish & Shellfish Immunology·Youhua HuangQiwei Qin
Aug 12, 2015·Fish & Shellfish Immunology·Tianle GuNana Yan
Dec 28, 2011·Developmental and Comparative Immunology·K V RajendranZhanjiang Liu
Dec 3, 2014·Developmental and Comparative Immunology·Xiaohui ChenTianle Gu
Jul 6, 2018·The Journal of Veterinary Medical Science·Ryota KobaYukinobu Tohya

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