Molecular cloning and localization of the luteinizing hormone beta subunit and glycoprotein hormone alpha subunit from Japanese anchovy Engraulis japonicus

Journal of Fish Biology
M OhkuboA Shimizu

Abstract

Although Clupeiformes contain many economically important species, there is limited information on their reproductive physiology. To obtain more insight into reproductive mechanisms in clupeiform fishes, molecular cloning of the Japanese anchovy Engraulis japonicus luteinizing hormone beta (LHbeta) and glycoprotein hormone alpha (GPHalpha) subunits, and immunocytochemistry of gonadotrophs in the pituitary using antisera raised against the synthetic peptides for both subunits were carried out. The cDNAs for LHbeta and GPHalpha subunits consisted of 963 and 535 nucleotides encoding 141 and 122 amino acids, respectively. The deduced amino acid sequences of the E. japonicus LHbeta subunit showed a 60% similarity to the Pacific herring Clupea pallasii LHbeta subunit and 24-31% similarities to FSHbeta subunits of other fish species. The E. japonicus GPHalpha subunit showed 52-57% similarities to anguilliform and cypriniform GPHalpha subunits. Both the subunits have typical structural characteristics of each subunit such as N-linked glycosylation sites, conserved cysteine residues and highly conserved short amino acid sequences. These results indicate that cDNAs cloned in this study encode the E. japonicus LHbeta and GPHalpha subunits...Continue Reading

References

Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S SekineS Itoh
Sep 1, 1988·General and Comparative Endocrinology·H ItohH Kawauchi
Sep 1, 1988·General and Comparative Endocrinology·K SuzukiH Kawauchi
Sep 1, 1988·General and Comparative Endocrinology·K SuzukiH Kawauchi
Dec 1, 1988·International Journal of Peptide and Protein Research·Y S ChangT B Lo
Nov 1, 1967·Comparative Biochemistry and Physiology·B Sullivan, A Riggs
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
May 1, 1983·General and Comparative Endocrinology·M Olivereau, Y Nagahama
Apr 1, 1996·General and Comparative Endocrinology·A ElizurY Zohar
Mar 30, 1999·General and Comparative Endocrinology·Y YoshiuraK Aida
Apr 29, 2000·Endocrine·A Ulloa-AguirreJ A Dias
Jul 10, 2003·General and Comparative Endocrinology·Raul Laiz-CarriónJuan Miguel Mancera
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Dec 3, 2014·General and Comparative Endocrinology·François ChauvignéJoan Cerdà

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