PMID: 9434110Jan 20, 1998Paper

Molecular cloning and N-terminal analysis of bovine cystatin C. Identification of a full-length N-terminal region

Biochimica Et Biophysica Acta
S L OlssonI Björk

Abstract

The N-terminal region of human cystatin C has been shown to be of crucial importance for the interaction of the inhibitor with cysteine proteinases. However, several studies have been unable to identify the corresponding region in bovine cystatin C, indicating that the binding of proteinases to the bovine inhibitor may not be dependent on this region. With the aim to resolve this apparent discrepancy and to elucidate the relation of bovine cystatin C to other cystatins, we have isolated a cDNA clone encoding bovine precystatin C. The sequence of this cDNA was similar to that of the human inhibitor and showed a putative signal peptidase cleavage site consistent with the N-terminal regions of the bovine and human inhibitors being of comparable size. This suggestion was verified by determination of the relative molecular mass of the mature bovine inhibitor isolated from cerebrospinal fluid under conditions minimising proteolysis. The N-terminal of the purified inhibitor was blocked, but the sequence of the N-terminal peptide produced by digestion with endopeptidase LysC could be unequivocally determined by tandem mass spectroscopy. Together, these results show that bovine cystatin C has 118 residues, in contrast with 110-112 resid...Continue Reading

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Citations

Jul 7, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K R NicholasD C Shaw
Feb 25, 2003·Bioscience, Biotechnology, and Biochemistry·Yasuhiro MatsuokaMasayoshi Kumegawa
May 8, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Richard E GoodmanGavin P Thompson

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