PMID: 9658017Jul 11, 1998Paper

Molecular cloning and nucleotide sequence of the superoxide dismutase gene and characterization of its product from Bacillus subtilis

Journal of Bacteriology
T InaokaT Tsuchido

Abstract

Bacillus subtilis was found to possess one detectable superoxide dismutase (Sod) in both vegetative cells and spores. The Sod activity in vegetative cells was maximal at stationary phase. Manganese was necessary to sustain Sod activity at stationary phase, but paraquat, a superoxide generator, did not induce the expression of Sod. The specific activity of purified Sod was approximately 2, 600 U/mg of protein, and the enzyme was a homodimer protein with a molecular mass of approximately 25,000 per monomer. The gene encoding Sod, designated sodA, was cloned by the combination of several PCR methods and the Southern hybridization method. DNA sequence analysis revealed the presence of one open reading frame consisting of 606 bp. Several putative promoter sites were located in the upstream region of sodA. The deduced amino acid sequence showed high homology with other bacterial manganese Sods. Conserved regions in bacterial manganese Sod could also be seen. The phenotype of double mutant Escherichia coli sodA sodB, which could not grow in minimal medium without supplemental amino acids, was complemented by the expression of B. subtilis sodA.

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Citations

Oct 3, 2003·Microbiology·Michail H KaravolosSimon J Foster
May 5, 2009·The Journal of Microbiology·Na-Rae Yun, Young Nam Lee
Jun 20, 2020·Microbial Biotechnology·Anne Soisig SteunouSoufian Ouchane
Apr 6, 2005·Molecular Genetics and Genomics : MGG·Torsten KoburgerM Hecker
Oct 14, 2010·Folia Microbiologica·D PetráčkováJ Svobodová
Jun 29, 2014·Applied and Environmental Microbiology·Fernando Santos-EscobarMario Pedraza-Reyes
May 10, 2017·Bioscience, Biotechnology, and Biochemistry·Takashi InaokaKazutaka Yamamoto
Nov 23, 2006·Journal of Bacteriology·Helena B ThomaidesLloyd Czaplewski
Aug 30, 2001·Applied and Environmental Microbiology·C BarrièreR Talon
Feb 20, 2021·Frontiers in Microbiology·Holly Anne MartinEduardo A Robleto
Sep 3, 2021·Research in Microbiology·Valentin WasselinEliette Riboulet-Bisson

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