Molecular cloning and nucleotide sequence analysis of mRNA for human kidney ornithine aminotransferase. An examination of ornithine aminotransferase isozymes between liver and kidney

FEBS Letters
T KobayashiT Matsuzawa

Abstract

The cDNA encoding ornithine aminotransferase (EC 2.6.1.13; OAT) was isolated from a human kidney cDNA library. The isolated cDNA contained the entire protein coding region and partial 3'- and 5'-untranslated regions. The nucleotide sequences of human kidney OAT cDNA were absolutely homologous with those of human liver OAT cDNA, and human kidney and liver OAT fused completely against the antibody to human kidney OAT in an Ouchterlony double diffusion test. These findings settled the controversy as to which characteristics of liver and kidney OAT isozymes are different. An N-terminal sequence analysis of purified mature human kidney OAT clarified that the leader peptide was cleaved between Gln-35 and Gly-36.

References

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Citations

Jul 1, 2009·Indian Journal of Clinical Biochemistry : IJCB·H Ravi KumarH P Puttaraju
Oct 1, 1993·Molecular and Biochemical Parasitology·S R SchmidH A Stanley
Apr 16, 2013·Antioxidants & Redox Signaling·Xinwen LiangDonald F Becker
Apr 29, 1998·Journal of Molecular Biology·B W ShenT Schirmer
Aug 3, 2010·Journal of Molecular Biology·Esther JortzikKatja Becker
Feb 23, 2012·The Korean Journal of Parasitology·Aleksandro S Da SilvaSilvia G Monteiro
Oct 18, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Riccardo MontioliBarbara Cellini

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