Molecular cloning and pharmacological characterization of two novel GnRH receptors in the lamprey (Petromyzon marinus).

Endocrinology
Nerine T JosephStacia A Sower

Abstract

This paper reports the identification, expression, binding kinetics, and functional studies of two novel type III lamprey GnRH receptors (lGnRH-R-2 and lGnRH-R-3) in the sea lamprey, a basal vertebrate. These novel GnRH receptors share the structural features and amino acid motifs common to other known gnathostome GnRH receptors. The ligand specificity and activation of intracellular signaling studies showed ligands lGnRH-II and -III induced an inositol phosphate (IP) response at lGnRH-R-2 and lGnRH-R-3, whereas the ligand lGnRH-I did not stimulate an IP response. lGnRH-II was a more potent activator of lGnRH-R-3 than lGnRH-III. Stimulation of lGnRH-R-2 and lGnRH-R-3 testing all three lGnRH ligands did not elicit a cAMP response. lGnRH-R-2 has a higher binding affinity in response to lGnRH-III than lGnRH-II, whereas lGnRH-R-3 has a higher binding affinity in response to lGnRH-II than IGnRH-III. lGnRH-R-2 precursor transcript was detected in a wide variety of tissues including the pituitary whereas lGnRH-R-3 precursor transcript was not as widely expressed and primarily expressed in the brain and eye of male and female lampreys. From our phylogenetic analysis, we propose that lGnRH-R-1 evolved from a common ancestor of all verte...Continue Reading

References

Jul 1, 1992·Molecular Endocrinology·M TsutsumiS C Sealfon
Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1988·Computer Applications in the Biosciences : CABIOS·E W Myers, W Miller
Dec 1, 1996·Computer Applications in the Biosciences : CABIOS·N GaltierC Gautier
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·L WangH B Kwon
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Apr 15, 2004·Endocrine Reviews·Robert P MillarStuart R Maudsley
Nov 25, 2004·General and Comparative Endocrinology·Kevin Morgan, Robert P Millar
Jun 16, 2005·Journal of Molecular Endocrinology·Natalia MoncautAdelino V M Canário
Sep 6, 2005·Animal Reproduction Science·Robert P Millar
Sep 15, 2005·The Journal of Biological Chemistry·Perdita E BarranRobert P Millar
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Mar 7, 2009·Endocrinology·Javier A Tello, Nancy M Sherwood
Aug 7, 2009·Neuroendocrinology·Alan J StewartKevin Morgan
Aug 17, 2010·General and Comparative Endocrinology·Stacia A SowerScott I Kavanaugh
Nov 1, 2008·Integrative and Comparative Biology·Javier A TelloNancy M Sherwood

❮ Previous
Next ❯

Citations

Aug 12, 2014·General and Comparative Endocrinology·Graeme J RochNancy M Sherwood
Jun 13, 2013·General and Comparative Endocrinology·Nerine T JosephRobert P Millar
Jun 19, 2016·General and Comparative Endocrinology·David C PlachetzkiStacia A Sower
Jun 18, 2017·General and Comparative Endocrinology·Meet ZandawalaMaurice R Elphick
Sep 22, 2017·Frontiers in Endocrinology·Tsubasa SakaiHonoo Satake
Aug 8, 2017·General and Comparative Endocrinology·Emily R Van GulickStacia A Sower

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.