PMID: 1903654Jun 4, 1991Paper

Molecular cloning and primary structure of rat thyroxine-binding globulin

Biochemistry
S ImamuraS Refetoff

Abstract

Rat thyroxine-binding globulin (TBG) cDNAs were isolated from a rat liver cDNA library by using a human TBG cDNA as a probe. From two overlapping cDNA inserts, an aligned cDNA sequence of 1714 nucleotides was obtained. There was 70% homology with human TBG cDNA over the span of 1526 nucleotides. In order to confirm that the cloned cDNA encodes rat TBG and to localize the NH2-terminal amino acid of the mature molecule, the protein was purified by affinity chromatography and subjected to direct protein microsequencing. The NH2-terminal amino acid sequence was identical with that deduced from the nucleotide sequence. The rat TBG cDNA sequenced consisted of a truncated leader sequence (35 nucleotides), the complete sequence encoding the mature protein (1194 nucleotides) and the 3'-untranslated region (485 nucleotides), containing two polyadenylation signals. It was deduced that rat TBG consists of 398 amino acids (Mr = 44,607), three NH2-terminal residues more than human TBG, with which it shares 76% homology in primary structure. Of the six potential N-glycosylation sites, four are located in conserved positions compared to human TBG. Northern blot analysis of rat liver revealed an approximately 1.8-kilobase TBG mRNA. Its amount i...Continue Reading

References

Jan 1, 1976·Endocrinology·R L Sutherland, M R Brandon
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1990·Molecular Endocrinology·Y MurataN Matsui
Nov 13, 1987·Biochemical and Biophysical Research Communications·L SavuE A Nunez
Feb 27, 1989·FEBS Letters·R VranckxE A Nunez
Mar 1, 1985·The Journal of Clinical Endocrinology and Metabolism·Y MurataS Refetoff
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I L FlinkE Morkin
Nov 3, 1988·Nature·Y Amemiya, J Miyahara
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G L HammondC W Bardin
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Jan 1, 1980·The Journal of Endocrinology·H Garcin, P Higueret

Citations

Feb 23, 1995·Molecular and Cellular Biochemistry·M Yamaguchi, K Oishi
Dec 1, 1993·Molecular and Cellular Endocrinology·A Tsykin, G Schreiber
Feb 19, 2002·Molecular and Cellular Endocrinology·O E JanssenRalf Einspanier
Feb 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G Schreiber, S J Richardson
Jan 1, 1992·Thyroid : Official Journal of the American Thyroid Association·L Bartalena, J Robbins
Jan 28, 2004·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Neepa Y ChoksiMichael Shelby
May 1, 1993·Journal of Endocrinological Investigation·L Bartalena

Related Concepts

DNA, Double-Stranded
Protein Glycosylation
Liver
August Rats
Poly(A) Tail
Homologous Sequences, Nucleic Acid
Novothyral
Thyroxine-Binding Proteins
Restriction Mapping
Whole Genomic DNA Probes

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