Molecular cloning and sequence analysis and the response of a aryl hydrocarbon receptor homologue gene in the clam Ruditapes philippinarum exposed to benzo(a)pyrene

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Na LiuLin Zhang

Abstract

The aryl hydrocarbon receptor (AhR) is a member of the basic helix-loop-helix/Per-Arnt-Sim (bHLH/PAS) family of transcription factors. In the present study, a cDNA encoding an AhR homologue was initially cloned and sequenced from the clam, Ruditapes philippinarum. The predicted amino acid sequences contain regions characteristic of AhRs from other species including basic helix-loop-helix (bHLH) and Per-ARNT-Sim (PAS) domains, but it does not contain distinct Q-rich domain as found in the mammalian AhRs. The clam AhR homologue cDNA coded for a 765 amino acids protein and phylogenetic analysis demonstrated that it was clustered within the invertebrate AhRs branch. The clam AhR homologue mRNA expression was detected in all the adult tissues tested (gill, digestive gland, adductor muscle and mantle) and highest transcription level were observed in gill compared to other tissues. Quantitative real-time RT-PCR analysis revealed that the clam AhR homologue mRNA expression levels in gill and digestive gland of R. philippinarum were induced by benzo(a)pyrene (BaP) and the absolute expression levels of the gene showed temporal and dose-dependent response.

References

Dec 8, 1995·The Journal of Biological Chemistry·B N FukunagaO Hankinson
Jan 13, 1994·Biochemical Pharmacology·D W Nebert
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M DolwickC A Bradfield
Feb 16, 1996·The Journal of Biological Chemistry·B N Fukunaga, O Hankinson
Sep 1, 1996·Toxicology and Applied Pharmacology·P M Fernandez-SalgueroF J Gonzalez
Dec 6, 1996·The Journal of Biological Chemistry·H I Swanson, J h Yang
Jan 1, 1996·Annual Review of Cell and Developmental Biology·J V Schmidt, C A Bradfield
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·W R Atchley, W M Fitch
Aug 15, 1997·Archives of Biochemistry and Biophysics·N K Roy, I Wirgin
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J A Powell-CoffmanW B Wood
Feb 5, 1999·Biochimica Et Biophysica Acta·R L TanguayR E Peterson
Aug 2, 2003·Biochemical and Biophysical Research Communications·Hiroaki OhiShigesada Higuchi
Nov 10, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rondi A ButlerRebecca J Van Beneden
Jul 12, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Masanobu YamauchiShinsuke Tanabe

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Citations

Jul 4, 2012·Environmental Science and Pollution Research International·Ali KaramiSimon C Courtenay
Mar 2, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuefeng CaiJingjing Miao
Oct 8, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Zhongyuan GaoLuqing Pan

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