PMID: 2888479Jun 16, 1987

Molecular cloning and sequence analysis of cDNAs encoding porcine kidney D-amino acid oxidase

Biochemistry
K FukuiY Miyake

Abstract

Complementary DNAs encoding D-amino acid oxidase (EC 1.4.3.3, DAO), one of the principal and characteristic enzymes of the peroxisomes of porcine kidney, have been isolated from the porcine kidney cDNA library by hybridization with synthetic oligonucleotide probes corresponding to the partial amino acid sequences. Analysis of the nucleotide sequences of two clones revealed a complete 3211-nucleotide sequence with a 5'-terminal untranslated region of 198 nucleotides, 1041 nucleotides of an open reading frame that encoded 347 amino acids, and a 3'-terminal untranslated region of 1972 nucleotides. The deduced amino acid sequence was completely identical with the reported sequence of the mature enzyme [Ronchi, S., Minchiotti, L., Galliano, M., Curti, B., Swenson, R. P., Williams, C. H. J., & Massey, V. (1982) J. Biol. Chem. 257, 8824-8834]. These results indicate that the primary translation product does not contain a signal peptide at its amino-terminal region for its translocation into peroxisomes. RNA blot hybridization analysis suggests that porcine kidney D-amino acid oxidase is encoded by three mRNAs that differ in size: 3.3, 2.7, and 1.5 kilobases. Comparison of the sequences of the two cDNA clones revealed that multiple pol...Continue Reading

References

Feb 7, 2007·Applied Microbiology and Biotechnology·Birgit GeuekeWerner Hummel
Mar 30, 2010·Applied Microbiology and Biotechnology·Zhanglin Lin, Jing Zheng
Feb 13, 2009·Amino Acids·Minoru TanigawaYoko Nagata
Jan 1, 1991·The International Journal of Biochemistry·R KonnoY Yasumura
Apr 1, 1992·The International Journal of Biochemistry·R Konno, Y Yasumura
Dec 15, 1988·Biochimica Et Biophysica Acta·R KonnoY Yasumura
Apr 13, 1995·Biochimica Et Biophysica Acta·B Schilling, K Lerch
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M KatagiriT Yamano
Aug 25, 1992·Biochimica Et Biophysica Acta·M SasakiJ Enami
Jul 1, 1997·Progress in Neurobiology·Atsushi Hashimoto, T Oka
Sep 30, 2009·Molecular Psychiatry·L VerrallP J Harrison
Mar 1, 1989·European Journal of Biochemistry·M Pilone SimonettaS Ronchi
Mar 10, 2009·Biochemistry. Biokhimii︠a︡·A Gholizadeh, B B Kohnehrouz
Feb 9, 2000·Bioscience, Biotechnology, and Biochemistry·E TakahashiT Yoshimoto
Sep 3, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hiroki SomaYoko Nagata
Oct 10, 2007·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Tomoya KawazoeKiyoshi Fukui
Oct 26, 2005·Biotechnology and Bioengineering·Mikio BakkeNaoki Kajiyama
Dec 5, 2006·The FEBS Journal·Masumi KataneHiroshi Homma
Jun 15, 1989·Biochemical and Biophysical Research Communications·E CiccarelliA Bollen
Nov 9, 2004·Brain Research. Molecular Brain Research·Masanobu YoshikawaAtsushi Hashimoto
Oct 22, 2003·Archives of Biochemistry and Biophysics·Xinle Wu, V M Monnier
Jun 22, 1999·DNA Sequence : the Journal of DNA Sequencing and Mapping·R KonnoA Niwa

Citations

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G A HamiltonK W Zerby
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·B M Goldman, G Blobel
Dec 30, 1986·Biochemical and Biophysical Research Communications·K FukuiY Miyake
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S FurutaT Hashimoto
Mar 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·M J Cline, R I Lehrer
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Feb 1, 1984·Journal of Neurochemistry·R Konno, Y Yasumura
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J C Wallace, M Edmonds
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Jun 1, 1980·Gene·D Hanahan, M Meselson
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Mar 18, 1961·Biochimica Et Biophysica Acta·V MASSEYR BENNETT

Related Concepts

D-Amino Acid Dehydrogenase
Untranslated Regions
Enzymes, antithrombotic
DAO gene
Nucleic Acid Hybridization Procedure
Amino Acids, I.V. solution additive
Oligonucleotide Probes
Kidney
Sense Codon
Sequence Analysis

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.