Molecular cloning and sequence analysis of full-length cDNA coding for mouse contrapsin

Journal of Biochemistry
Y SuzukiH Sinohara

Abstract

Four overlapping cDNA clones encoding contrapsin were isolated from a mouse liver cDNA library constructed in the expression vector, lambda gt11. M13 vector sequence analysis revealed that contrapsin cDNA contained an open reading frame of 1,254 bases encoding 418 amino acids. The N-terminal amino acid sequence of the isolated contrapsin matched residues 30 to 48 of the sequence deduced on nucleotide analysis. One clone, which had the longest 3' untranslated region, contained two sets of tandem polyadenylation signals, AATACA and AATAAA, which were located 497 bases apart, while the remaining three clones terminated at the first signal. The entire reading frame sequence of contrapsin cDNA showed 64% homology with that of human alpha-1-antichymotrypsin.

References

Mar 28, 2002·DNA Sequence : the Journal of DNA Sequencing and Mapping·K YoshidaH Sinohara
Aug 18, 2004·Nature Immunology·Ni LiuPhilip G Ashton-Rickardt
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P D'Eustachio

Related Concepts

SERPINA3 gene
Trypsin Inhibitors
expression vector
Amino Acids, I.V. solution additive
Polyadenylation Pathway
Contrapsin
Clone
Sequence Analysis
Genetic Vectors
Alpha 1-Antichymotrypsin

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