PMID: 2116136Jun 1, 1990Paper

Molecular cloning and sequence analysis of the cDNA for the putative beta subunit of the type-II gonadotrophin from the European eel

Journal of Molecular Endocrinology
B QuératR Counis

Abstract

Oestradiol treatment enhances type-II gonadotrophin (GTH-II) synthesis in the European eel (Anguilla anguilla) at the silver stage. As a first step in studying the molecular mechanisms involved in this stimulation, we cloned and characterized the cDNA encoding the beta subunit of eel GTH-II. A cDNA library was constituted in lambda gt10 from oestradiol-treated eels. It was screened using an oligodeoxyribonucleotide mixed probe designed to be complementary to a highly conserved region of cDNAs from several LH-related beta subunits. Several clones were obtained and four were subcloned in pUC13 and sequenced. The longest clones comprised a 420 bp coding sequence, plus 5' and 3' untranslated regions of 36 and 172 bp respectively. Comparison with GTH-II from other teleost fish permitted the localization of the putative cleavage site of a 24 amino acid signal peptide. The resulting 116 amino acid apopeptide had well-conserved cysteine positions and a putative N-linked glycosylation site; homology was 70-80% with GTH-II from other fish, 45% with LH from mammals and birds, 38% with mammalian FSH and only 35% with fish GTH-I. Preliminary results indicated a strong positive effect of oestradiol treatment on the level of the putative GTH-...Continue Reading

Citations

Aug 8, 1997·Molecular and Cellular Endocrinology·Y S HuangS Dufour
Oct 8, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G DeganiK Jackson
Dec 24, 2014·General and Comparative Endocrinology·Adimoolam ArunaChing-Fong Chang
Dec 13, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nozomi WatanabeSatoshi Hayakawa
Jan 1, 1994·International Journal of Peptide and Protein Research·T OkadaH Kawauchi
Dec 4, 2019·General and Comparative Endocrinology·G MaugarsK Rousseau
Jun 1, 1994·Molecular and Cellular Endocrinology·B QuératG D'Angelo

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