PMID: 9418242Jan 7, 1998Paper

Molecular cloning and sequence analysis of the lysR gene from the extremely thermophilic eubacterium, Thermus thermophilus HB27

FEMS Microbiology Letters
T Kosuge, T Hoshino

Abstract

We have isolated a lysine-auxotrophic and kanamycin-resistant mutant from an extreme thermophile, Thermus thermophilus HB27. This mutant showed the lysA- or lysR- genotype since it could not grow on the minimal plate which contained diaminopimelic acid. Sequence analysis of the clones which could rescue the Lys- mutant indicated the lysR gene. The lysR gene overlapped with the rimK gene for the modification enzyme of ribosomal protein S6. In the Lys- mutant, the lysR gene was disrupted and the C-terminus region of the RimK protein was different from that of the wild-type, which contributed to the Lys- and kanamycin-resistant phenotype. The deduced amino acid sequence of the lysR gene showed 20.9% identity with the LysR protein of Escherichia coli. The percentage of use of cytosine or guanine in the third letter of the codons in the lysR gene was only 67.4%. We also determined that the argC gene encoding N-acetyl-gamma-glutamyl phosphate reductase and the argB gene encoding acetylglutamate kinase were located immediately upstream of the lysR gene.

References

Dec 1, 1992·Journal of Bacteriology·T HeinrichR K Hartmann
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M E MaxonH Weissbach
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jul 1, 1965·The Biochemical Journal·P J WHITE, B KELLY

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Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·T Kosuge, T Hoshino
Sep 17, 2011·Bioorganic Chemistry·Maria V FawazSteven M Firestine
Sep 10, 2003·Journal of Molecular Biology·Hiroaki SakaiShigeyuki Yokoyama
May 15, 2002·FEMS Microbiology Reviews·Roy D Sleator, Colin Hill
Dec 30, 1998·FEMS Microbiology Letters·T Kosuge, T Hoshino
Oct 26, 2010·Journal of Molecular Biology·Isabel Pérez-ArellanoJavier Cervera

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