PMID: 9550267Apr 29, 1998Paper

Molecular cloning and sequence analysis of the aggrecan interglobular domain from porcine, equine, bovine and ovine cartilage: comparison of proteinase-susceptible regions and sites of keratan sulfate substitution

Matrix Biology : Journal of the International Society for Matrix Biology
Carl R FlanneryB Caterson

Abstract

Oligonucleotide primers which were designed based on identical peptide sequences flanking the interglobular domain (IGD) of human, bovine and rat aggrecan were used in RT-PCR reactions containing human, porcine, equine, bovine and ovine cartilage RNA. Novel cDNAs encoding the IGD of the latter four species were obtained and sequenced. The deduced amino acid sequences for these cDNAs were aligned and compared with those described for six other species. Amino acid sequences surrounding the major proteolytic cleavage sites in the IGD are highly conserved, with some species-specific substitutions. Similarly, known sites of keratan sulfate attachment in the IGD are highly conserved in all species. The results provide essential amino acid sequence data for species commonly used in model systems of cartilage degeneration.

References

Aug 15, 1992·Archives of Biochemistry and Biophysics·C FlanneryJ Sandy
Feb 12, 1996·FEBS Letters·A J FosangP J Neame
Oct 6, 1997·Archives of Biochemistry and Biophysics·T M HeringT D Huynh
Aug 15, 1997·The Biochemical Journal·R SztrolovicsJ S Mort

❮ Previous
Next ❯

Citations

Jul 17, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Mauro AliniHans Joachim Wilke
Aug 30, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·B CatersonC B Little
May 16, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Christopher B LittleBruce Caterson
May 18, 2001·Biochemical and Biophysical Research Communications·M SabatiniJ Bonnet
Oct 5, 2011·Connective Tissue Research·Megan S LordJohn M Whitelock
Feb 25, 2015·Arthritis & Rheumatology·Sophie LeesAmanda J Fosang
Apr 30, 2008·Methods : a Companion to Methods in Enzymology·Anthony J HayesBruce Caterson
Oct 9, 2014·Advanced Healthcare Materials·Stacey C SkaalureStephanie J Bryant
Oct 31, 2002·Equine Veterinary Journal. Supplement·C Palmgren KarlssonJ E Lindberg
Mar 20, 1998·Arthritis and Rheumatism·C Vingsbo-LundbergR Holmdahl
Mar 23, 2018·JCI Insight·Rachel E MillerAnne-Marie Malfait
Jul 15, 1999·Biochemical and Biophysical Research Communications·C R FlanneryB Caterson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.