Molecular cloning and sequence determination of a cDNA coding for the alpha-subunit of a Go-type protein of Xenopus laevis oocytes

FEBS Letters
J OlateJ E Allende

Abstract

Xenopus laevis oocytes are cells ideally suited to the study of signal transduction and of the G-proteins that are involved in this process. A X. laevis cDNA library in lambda gt10 has been screened with a mixture of three oligonucleotide probes designed to detect sequences found in various mammalian alpha-subunits of G-proteins. One of these clones has been purified through tertiary screening and the DNA insert has been sequenced. This clone was found to include the total sequence coding for a 354 amino acid protein that is 89% identical to the sequence of alpha-subunit of rat Go. The differences with the mammalian protein were clustered in amino acids 290-315, which have been postulated to define the region interacting with the receptor and effector molecule. The homology with the alpha-subunits of other mammalian G-proteins is lower (65-70% to Gi and 42% to Gs). On this basis, this clone can be classified as Go-like.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1987·Molecular and Cellular Endocrinology·A M Spiegel
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W MeyerhofD Richter
May 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Allende
Nov 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A A SmithG Brooker
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N DascalY Lass
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H ItohK Suzuki
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H K FongM I Simon
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J N DholakiaA J Wahba
Aug 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C B GundersenI Parker
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K SumikawaR Miledi

❮ Previous
Next ❯

Citations

Dec 1, 1994·Plant Molecular Biology·A L Drayer, P J van Haastert
Jan 1, 1991·Pharmacology & Therapeutics·J Olate, J E Allende
May 7, 1990·Biochimica Et Biophysica Acta·L BirnbaumerA M Brown
May 1, 1995·Cellular Signalling·L Paquereau, Y Audigier
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T TsukamotoY Kaziro
Dec 6, 1996·The Journal of Biological Chemistry·T M FiltzT K Harden
Feb 24, 1995·The Journal of Biological Chemistry·P de la PeñaF Barros
Jul 25, 1998·The Journal of Biological Chemistry·H ShapiraJ F Battey
Dec 15, 1995·The Journal of Biological Chemistry·L Stehno-BittelD E Clapham
Mar 15, 1991·Biochemical and Biophysical Research Communications·K SpicherW Rosenthal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.