Molecular cloning and sequencing of a cDNA for an auxin-repressed mRNA: correlation between fruit growth and repression of the auxin-regulated gene

Plant Molecular Biology
A S Reddy, B W Poovaiah

Abstract

A complementary DNA (cDNA) library has been constructed in lambda gt 10 from poly(A)+ mRNA isolated from auxin-deprived strawberry receptacles. By differential plaque filter hybridization, a cDNA (lambda SAR5) to an auxin-repressed mRNA has been isolated. The expression of the auxin-repressed gene is studied at various stages of normal fruit development and in fruits of variant strawberry genotype using lambda SAR5 as a probe. Northern analyses of RNA isolated from pollinated and unpollinated fruits of various developmental stages revealed that mRNA corresponding to the lambda SAR5 clone is repressed during normal fruit development, and the level of lambda SAR5 mRNA is regulated by endogenous auxin. Furthermore, results with both normal and variant genotype strawberry fruit indicate that there is a positive correlation between growth of strawberry fruit and repression of mRNA corresponding to the lambda SAR5 clone. The lambda SAR5 cDNA has been sequenced and is 723 nucleotides in length. The deduced protein has 111 amino acid residues with a molecular mass of 12.5 kDa. The putative polypeptide starts at nucleotide position 20 and ends at 352. The molecular weight of the predicted polypeptide is in agreement with the molecular w...Continue Reading

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Citations

Dec 1, 1994·Plant Molecular Biology·L HobbieM Estelle
Nov 1, 1993·Plant Physiology·S A LeeM Lay-Yee
Dec 14, 1999·Plant Physiology·P M CivelloA B Bennett
Feb 1, 1992·Plant Physiology·D BreviarioI Coraggio
Nov 1, 1992·The Plant Journal : for Cell and Molecular Biology·A GranellJ Carbonell
Jul 18, 2012·Genetics and Molecular Biology·Maria Eugênia Lisei de SáMaria Fátima Grossi de Sá
Jan 1, 1993·Critical Reviews in Plant Sciences·B W Poovaiah, A S Reddy

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