PMID: 9529053Apr 7, 1998Paper

Molecular cloning and sequencing of three granulocytic Ehrlichia genes encoding high-molecular-weight immunoreactive proteins

Infection and Immunity
J R StoreyC I Murphy

Abstract

Granulocytic Ehrlichia was isolated from canine blood obtained from animals challenged with field-collected Ixodes scapularis and propagated in HL60 cells. PCR primers specific for the 16S ribosomal DNA (rDNA) of the Ehrlichia genogroup comprising E. equi, E. phagocytophila, and the agent of human granulocytic ehrlichiosis (HGE) amplified DNA from extracts of these cells. Sequence analysis of this amplified DNA revealed that it is identical to the 16S rDNA sequence of the HGE agent. A genomic library was constructed with DNA from granulocytic Ehrlichia and screened with pooled sera from tick-challenged, granulocytic Ehrlichia-infected dogs. Several clones were isolated and sequenced. Three complete genes encoding proteins with apparent molecular masses of 100, 130, and 160 kDa were found. The recombinant proteins reacted with convalescent-phase sera from dogs and human patients recovering from HGE. This approach will be useful for identifying candidate diagnostic and vaccine antigens for granulocytic ehrlichiosis and aid in the classification of genogroup members.

References

Apr 1, 1992·Trends in Biochemical Sciences·V BlankA Israël
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D R AllredA F Barbet
Jan 1, 1992·Journal of Clinical Microbiology·Y RikihisaM B Malole
Dec 1, 1991·Journal of Clinical Microbiology·B E AndersonK H Wilson
Jul 1, 1991·Clinical Microbiology Reviews·Y Rikihisa
Sep 1, 1990·Journal of Clinical Microbiology·K H WilsonR C Greene
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Karlin, S F Altschul
Apr 1, 1989·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S J RodgersC A Baldwin
Jul 1, 1989·Infection and Immunity·D F HoftL V Kirchhoff
Jan 1, 1987·Annual Review of Microbiology·D J KempR F Anders
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Oct 1, 1995·The Journal of Infectious Diseases·J E MadiganJ S Dumler
Mar 1, 1994·Journal of Clinical Microbiology·S M ChenD H Walker
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Karlin, S F Altschul
Jan 25, 1996·The New England Journal of Medicine·J L GoodmanU G Munderloh
Oct 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C J HardaloJ S Dumler
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·S R TelfordD H Persing
Jan 1, 1996·Microbial Pathogenesis·M MatsumotoS Fujita
Nov 1, 1996·Journal of Clinical Microbiology·X J YuD H Walker
May 1, 1997·Journal of Clinical Microbiology·C P KolbertD H Persing
Apr 1, 1997·Journal of Clinical Microbiology·M T YehR F Massung
Jan 1, 1951·The Journal of Pathology and Bacteriology·A FOGGIE

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Citations

May 9, 2002·Emerging Infectious Diseases·Robert F MassungThomas N Mather
May 10, 2020·Parasites & Vectors·Denis B LangenwalderFriederike D von Loewenich
Mar 5, 2002·Clinical and Diagnostic Laboratory Immunology·Anneli BjöersdorffIngvar Eliasson
Jul 8, 2011·Clinical Microbiology Reviews·Yasuko Rikihisa
Oct 14, 2014·Cellular Microbiology·Andrea R BeyerJason A Carlyon
Jul 19, 2002·Molecular Microbiology·Inga Benz, M Alexander Schmidt
Jul 1, 2004·Expert Review of Molecular Diagnostics·J Stephen Dumler, Philippe Brouqui
Jul 31, 2010·Cellular Microbiology·Yasuko RikihisaHua Niu
Oct 8, 2016·Frontiers in Cellular and Infection Microbiology·Aminat T OkiJason A Carlyon
Aug 2, 2000·Journal of Clinical Microbiology·R F MassungC I Murphy
Feb 8, 2003·Journal of Clinical Microbiology·Robert F Massung, Kimetha G Slater
Nov 8, 2003·Journal of Clinical Microbiology·Friederike D von LoewenichChristian Bogdan
Jan 9, 2004·Clinics in Laboratory Medicine·J Stephen Dumler

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