Molecular cloning and tissue distribution of a short form chicken leptin receptor mRNA

Domestic Animal Endocrinology
Xiaojun LiuT Boswell

Abstract

In mammals, alternative splicing of the leptin receptor (LEPR) produces several C-terminal truncated isoforms that are believed to play a role in the transport, cellular internalisation and degradation of the hormone leptin. The chicken leptin receptor (chLEPR) is similar to its mammalian counterparts in terms of its intron/exon structure and conserved motifs. However, it is unknown whether the chLEPR also undergoes alternative splicing. To test this, structural analysis of intron 19 of the chLEPR, equivalent to the intron in which alternative splicing occurs in mammals, was combined with 3'-rapid amplification of cDNA ends (3'-RACE) to search for chLEPR splice variants. A 44-amino acid alternative exon 20 was identified that is spliced to generate a short isoform of the chLEPR (chLEPR-SF). Comparative sequence analysis of intron 19 identified two regions that are highly conserved between the chicken and mammals, indicating their possible importance as intronic elements in the regulation of alternative splicing of the LEPR in vertebrates. Tissue expression of the chLEPR-SF was lower and more restricted than that of the chLEPR long isoform. Collectively these data demonstrate that the chLEPR is alternatively spliced to produce a...Continue Reading

References

Dec 29, 1995·Cell·L A TartagliaR I Tepper
Feb 15, 1996·Nature·G H LeeJ M Friedman
Sep 1, 1996·Current Biology : CB·B D BennettW Matthews
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·H FeiJ M Friedman
Apr 3, 1998·Gene·M TaouisJ Simon
Sep 11, 1999·General and Comparative Endocrinology·M Friedman-EinatP J Sharp
Apr 13, 2000·Biochimica Et Biophysica Acta·T OhkuboK Nakashima
Jun 16, 2000·Molecular and Cellular Endocrinology·G HorevM Friedman-Einat
Nov 22, 2000·Animal Genetics·F PitelA Vignal
Nov 22, 2000·Animal Genetics·I C DunnI R Paton
Jun 26, 2003·Nucleic Acids Research·Scott SchwartzWebb Miller
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mark P Richards, Stephen M Poch
Jun 25, 2004·Nucleic Acids Research·Kelly A FrazerInna Dubchak
Dec 14, 2004·Nature·UNKNOWN International Chicken Genome Sequencing Consortium
Jun 3, 2005·Genome Research·Xiang H-F ZhangLawrence A Chasin

❮ Previous
Next ❯

Citations

Mar 24, 2012·Animal : an International Journal of Animal Bioscience·A R G Wylie
Jun 19, 2007·Poultry Science·M P Richards, M Proszkowiec-Weglarz
May 27, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Takeshi OhkuboShoei Sugita
May 2, 2013·American Journal of Physiology. Endocrinology and Metabolism·Yixin SuVictor M Pulgar
Apr 16, 2013·General and Comparative Endocrinology·Patrycja GoggaWlodzimierz Meissner
Apr 23, 2013·General and Comparative Endocrinology·J M CorneliusM Ramenofsky
Sep 8, 2015·General and Comparative Endocrinology·Dandan WangXiaojun Liu
Mar 19, 2008·Animal Reproduction Science·Yueqiang SongZhenghong Zuo
Jan 11, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiaojun Liu, Peter J Sharp
Jan 15, 2015·General and Comparative Endocrinology·Timothy Boswell, Ian C Dunn
Jun 18, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Rongjie LiRuqian Zhao
Jun 12, 2016·Developmental and Comparative Immunology·Claudio ProcacciniGiuseppe Matarese
Nov 22, 2016·Animal Science Journal = Nihon Chikusan Gakkaihō·Qian BanXiaojun Liu
Apr 1, 2017·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·S BogatyrevB Tzschentke
Sep 1, 2020·Animal Biotechnology·Mahmoud S El-TarabanyMohammed A El-Magd
Jul 17, 2020·Frontiers in Physiology·Charlene HanlonGrégoy Y Bédécarrats
Jun 17, 2011·Neuroendocrinology·Robert J DenverGraham C Boorse
Nov 9, 2019·Trends in Endocrinology and Metabolism : TEM·Miriam Friedman-Einat, Eyal Seroussi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Related Papers

Molecular and Cellular Endocrinology
G HorevMiriam Friedman-Einat
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Mark P Richards, Stephen M Poch
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Xiaojun Liu, Peter J Sharp
© 2021 Meta ULC. All rights reserved