Molecular cloning, by a novel approach, of a cDNA encoding a putative olfactory protein in the labial palps of the moth Cactoblastis cactorum

Gene
R Maleszka, G Stange

Abstract

We have used the CapFinder technology, without the library construction step, to amplify and clone full-length cDNAs expressed in the labial palps (CO2-sensing organs) of the moth Cactoblastis cactorum. The validity of our approach is exemplified by the sequence analysis of a 597-bp cDNA clone, designated CLP-1, that contains a 390-bp open reading frame (ORF) flanked by motifs characteristic to a full-length cDNA. The ORF in CLP-1 encodes a predicted polypeptide that is 47% identical to a novel protein, OS-D, found exclusively in the olfactory antennal segment of Drosophila melanogaster. Both CLP-1 and OS-D have primary structures that do not bear sequence similarity to any previously characterised proteins including odorant-binding proteins (OBPs) in vertebrates and pheromone-binding proteins (PBPs) in moths. Although they share features common to OBPs and PBPs, such as the presence of signal peptides and cysteine motifs, they clearly belong to a distinct class of olfactory proteins that appear to be unique to insects. The relative abundance of the CLP-1 message in the labial palps of females leads to the suggestion that this protein is involved in the CO2-sensing cascade. Our results suggest that the experimental procedure ca...Continue Reading

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Citations

Dec 22, 1999·Microscopy Research and Technique·G Stange, S Stowe
Jul 18, 2000·Archives of Insect Biochemistry and Physiology·J F PicimbonP Pelosi
Sep 16, 2000·Insect Biochemistry and Molecular Biology·S MarcheseP Pelosi
Oct 5, 2001·Insect Biochemistry and Molecular Biology·J F PicimbonH Breer
Mar 25, 2000·Insect Biochemistry and Molecular Biology·J F PicimbonJ Krieger
May 31, 2011·Journal of Proteome Research·Mao FengJianke Li
Jul 31, 2013·Nature Reviews. Genetics·Ehud ShapiroSten Linnarsson
Sep 17, 2002·European Journal of Biochemistry·Loïc BriandJean-Claude Penollet
Apr 20, 2013·Chemical Senses·Immacolata IovinellaPaolo Pelosi
Dec 22, 1999·Biochemical and Biophysical Research Communications·A ScaloniP Pelosi
Oct 8, 2013·International Journal of Biological Macromolecules·Xin YiGuoHua Zhong
Feb 14, 2007·Insect Biochemistry and Molecular Biology·Da-Ping GongZhong-Huai Xiang
Mar 15, 2005·Insect Biochemistry and Molecular Biology·M CalvelloP Pelosi
Dec 19, 2006·Insect Biochemistry and Molecular Biology·Sylvain ForêtRyszard Maleszka
Jun 11, 2002·Biochemical and Biophysical Research Communications·Liping BanPaolo Pelosi

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