Molecular cloning, characterization and expression analysis of a putative C-type lectin (Fclectin) gene in Chinese shrimp Fenneropenaeus chinensis

Molecular Immunology
Yi-Chen LiuJian-Hai Xiang

Abstract

Lectin is regarded as a potential molecule involved in immune recognition and phagocytosis through opsonization in crustacean. Knowledge on lectin at molecular level would help us to understand its regulation mechanism in crustacean immune system. A novel C-type lectin gene (Fclectin) was cloned from hemocytes of Chinese shrimp Fenneropenaeus chinensis by 3' and 5' rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA consists of 1482 bp with an 861 bp open reading frame, encoding 287 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids. It also contains two carbohydrate recognition domains/C-type lectin-like domains (CRD1 and CRD2), which share 78% identity with each other. CRD1 and CRD2 showed 34% and 30% identity with that of mannose-binding lectin from Japanese lamprey (Lethenteron japonicum), respectively. Both CRD1 and CRD2 of Fclectin have 11 amino acids residues, which are relatively invariant in animals' C-type lectin CRDs. Five residues at Ca2+ binding site 1 are conserved in Fclectin. The potential Ca2+/carbohydrate-binding (site 2) motif QPD, E, NP (Gln-Pro-Asp, Glu, Asn-Pro) presented in the two CRDs of Fclectin may support its ability to bind galactose-type ...Continue Reading

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Citations

Apr 16, 2010·Marine Biotechnology·T W Flegel, Kallaya Sritunyalucksana
Jan 21, 2011·The Journal of Biological Chemistry·Tomomitsu HatakeyamaHideaki Unno
Jun 6, 2013·Journal of Pathogens·Bestha LakshmiD V R Sai Gopal
Jan 26, 2016·Fish & Shellfish Immunology·Yunji XiuQingguo Meng
Oct 13, 2012·Fish & Shellfish Immunology·Anchalee TassanakajonSureerat Tang
Aug 7, 2012·Developmental and Comparative Immunology·Lingling WangLinsheng Song
Apr 10, 2012·Developmental and Comparative Immunology·Fuhua Li, Jianhai Xiang
May 9, 2012·Developmental and Comparative Immunology·Xian-Wei Wang, Jin-Xing Wang
Nov 1, 2011·Developmental and Comparative Immunology·Leilei WangLinsheng Song
Jul 20, 2010·Developmental and Comparative Immunology·Jialong YangLinsheng Song
Aug 4, 2009·Fish & Shellfish Immunology·Xian-Wei WangJin-Xing Wang
Aug 4, 2009·Developmental and Comparative Immunology·Wipasiri SoonthornchaiPikul Jiravanichpaisal
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