Molecular cloning, characterization and expression analysis of grass carp (Ctenopharyngodon idellus) NF45 (ILF2) cDNA, a subunit of the nuclear factor of activated T-cells (NF-AT)

Fish & Shellfish Immunology
Hui-Fang LinHui-Ju Wang

Abstract

NF45 (ILF2) and NF90 (ILF3) regulate the IL-2 gene transcription via interaction with the antigen receptor response element. Much work on NF45 has been done in human and mammals while little in fish. In the present study, we have cloned and characterized the full-length cDNA of NF45 in grass carp (Ctenopharyngodon idellus). The grass carp NF45 cDNA of 1563bp contains a short 5'UTR of 24bp, a 3'UTR of 375bp and an open reading frame of 1164bp coding for a protein of 387 aa with a predicted molecular mass of 42.8kDa. The encoded protein shares 86.3-96.7% identities to other homologues. RT-PCR was optimized to estimate the expression level of NF45 in grass carp. The results showed that NF45 is constitutively expressed in most selected tissues, including head kidney, spleen, heart, brain, liver, and gill, although low levels were observed in spleen, liver and gill. The ubiquitous expression of NF45 is consistent with a postulated role in gene regulation at the level of transcription. Stimulating the fish with PHA significantly up-regulated the expression of NF45 in most tissues examined, which potentially indicated that NF45 was involved in the immune responses triggered by PHA.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D St JohnstonM Jantsch
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G J NabelD Baltimore
Apr 1, 1988·Molecular and Cellular Biology·D B DurandG R Crabtree
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·K T ShawP G Hogan
Jan 1, 1994·Cytogenetics and Cell Genetics·P M KroiselP J de Jong
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·E V Rothenberg, S B Ward
Dec 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P MarcoulatosN C Vamvakopoulos
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·H J LiaoM B Mathews
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·C G LeeJ Hurwitz
Dec 26, 2001·Nucleic Acids Research·Laurent FalquetAmos Bairoch
Jan 24, 2002·Mechanisms of Development·L A López-FernándezJ del Mazo
Jan 30, 2002·Gene·Irene T RombelStephen Albert Johnston
Sep 13, 2002·Nature·Zhaolan ZhouRobin Reed
Nov 14, 2002·Molecular Biology of the Cell·Alexander ScherlJean-Jacques Diaz
Dec 31, 2002·Molecular Cell·Jaekyung ShimMichael Karin
Jan 2, 2003·The Journal of Biological Chemistry·Carole GwizdekCatherine Dargemont
Nov 14, 2003·The Journal of Biological Chemistry·Qingde WangKazuko Nishikura
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Apr 9, 2005·Experimental Cell Research·Guohua ZhaoPeter N Kao

❮ Previous
Next ❯

Citations

Jul 29, 2008·Developmental and Comparative Immunology·Ellen H StolteB M Lidy Verburg-van Kemenade
Oct 8, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sujie NiWeijian Guo
Aug 24, 2017·Disease Markers·Zi-Huan YinDong-Feng Yu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.