PMID: 8593531Oct 1, 1995Paper

Molecular cloning, characterization, and mapping of a full-length cDNA encoding human UDP-galactose 4'-epimerase

Biochemical and Molecular Medicine
N DaudeJ Reichardt

Abstract

Galactose metabolism in all organisms is catalyzed by three enzymatic steps: the galactokinase, galactose-1-phosphate uridyltransferase, and UDP galactose 4'-epimerase reactions. We report here the molecular cloning, characterization, and mapping of a full-length cDNA encoding human UDP-galactose 4'-epimerase (GALE). Our cDNA is 1488 bp long and matches the mRNA size of 1.5 kg detected in fibroblasts and lymphoblasts. The human GALE cDNA encodes a predicted protein of 348 amino acids with a molecular mass of 38,266. The human GALE enzyme is 87% identical to the rat protein, 53% identical to the homologous GAL10 protein from the yeast Kluyveromyces lactis, and 51% identical to the galE protein from the prokaryote Escherichia coli. This extraordinary degree of sequence identity has allowed us to build a homology model of the human protein based on the bacterial crystal structure. This predicted human structure is very similar to the E. coli galE enzyme, suggesting that both enzymes use similar mechanisms. The human gene encoding GALE maps, as expected, to a single locus on chromosome 1 and appears to be compact. The human GALE gene is structurally intact in 19 patients with epimerase-deficiency galactosemia, an inborn error of me...Continue Reading

Citations

Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·P Dörmann, C Benning
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael BoyceCarolyn R Bertozzi
Nov 23, 2005·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Hyung-Doo ParkJunghan Song
Mar 23, 1999·Archives of Disease in Childhood·J H WalterR MacFaul
Dec 9, 2008·Chemico-biological Interactions·James E BrayUdo Oppermann
May 20, 2003·FEBS Letters·David J Timson, Richard J Reece
Aug 13, 1998·Journal of Inherited Metabolic Disease·A AlanoT M Cowan
Jul 15, 2000·The Journal of Biological Chemistry·X F Huang, V Luu-The
Apr 12, 2002·The Journal of Biological Chemistry·Toshio NinomiyaKoji Kimata
Jun 23, 2015·Chembiochem : a European Journal of Chemical Biology·Eun Ju Kim
Nov 13, 2020·Chembiochem : a European Journal of Chemical Biology·Jeremias E G A Dold, Valentin Wittmann
Jan 9, 2021·Chemical Reviews·Junfeng MaGerald W Hart
Mar 9, 2005·Archives of Biochemistry and Biophysics·Maya KamaoToshio Okano
Jun 8, 2007·Biochimica Et Biophysica Acta·Shivani AgarwalAparna Dixit

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.