Molecular cloning, characterization and mRNA expression of six peroxiredoxins from Black carp Mylopharyngodon piceus in response to lipopolysaccharide challenge or dietary carbohydrate

Fish & Shellfish Immunology
Chenglong WuJinyun Ye

Abstract

Peroxiredoxin (Prx) belongs to a cellular antioxidant protein family that plays important roles in innate immune function and anti-oxidative capability. In the present study, six Prxs were cloned from Black carp Mylopharyngodon piceus (MpPrx) by homology cloning and rapid amplification of cDNA ends (RACE) techniques. There were 199, 197, 250, 260, 189 and 222 amino acids in six MpPrxs, respectively. BLAST analysis reveals that MpPrxs shares high identities and similar characteristics with other known Prxs from animals. The phylogenetic analysis evidenced three major subclasses corresponding to one-Cys-Prx (MpPrx6), typical two-Cys-Prx (MpPrx1-4) and atypical 2-Cys-Prx (MpPrx5) that reflected the present hierarchy of vertebrates and invertebrates. Although six MpPrxs are constitutively expressed in all tissues, relatively higher-level mRNA expression levels of six MpPrxs can be detected in liver, eyes, heart and adipose tissues by real-time PCR assays. The transcriptional patterns of six MpPrxs mRNA in liver were detected by real-time PCR in Black carp after lipopolysaccharide (LPS) challenge and treated with graded levels of dietary carbohydrate (CHO) (106.5, 194.3, 288.4 and 379.1 g kg(-1)), respectively. These results showed ...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·S J Pilkis, D K Granner
Jan 1, 1995·Annual Review of Immunology·R J Ulevitch, P S Tobias
Apr 30, 1998·Current Opinion in Cell Biology·T Finkel
Feb 19, 2000·Current Opinion in Immunology·C BogdanA Diefenbach
Dec 13, 2000·The Journal of Biological Chemistry·C H ParkT Ganz
Mar 10, 2001·Free Radical Research·B HalliwellM Whiteman
Feb 14, 2002·Molecular Immunology·Gennady Ermak, Kelvin J A Davies
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 18, 2002·American Journal of Physiology. Endocrinology and Metabolism·Annette M Chang, Jeffrey B Halter
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Apr 19, 2003·The Journal of Biological Chemistry·Janine KönigKarl-Josef Dietz
Apr 26, 2003·Science·Zachary A WoodP Andrew Karplus
Dec 10, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Gregory LeyensBernard Knoops
Dec 18, 2003·Annals of the New York Academy of Sciences·Kathryn HaskinsJennifer Kench
Jan 30, 2004·Diabetes·Katherine GreenMichael P Murphy
Apr 6, 2004·The International Journal of Biochemistry & Cell Biology·Peter J Klover, Robert A Mooney
Apr 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Geoffrey W ThorpeIan W Dawes
Jul 29, 2004·FEBS Letters·Marlène DubuissonBernard Knoops
Sep 1, 2004·Free Radical Biology & Medicine·David M PattwellMalcolm J Jackson
Mar 1, 2005·Cell·Robert S BalabanToren Finkel
Mar 23, 2005·Current Opinion in Cell Biology·Sue Goo RheeHyun Ae Woo
May 14, 2005·Free Radical Biology & Medicine·Yefim Manevich, Aron B Fisher
Feb 17, 2006·Respiratory Physiology & Neurobiology·Melissa A Smith, Michael B Reid
Feb 24, 2007·Biochemical and Biophysical Research Communications·Lianqin LiMasuo Obinata
Mar 27, 2007·Free Radical Biology & Medicine·Bridget M Gallagher, Shelley A Phelan
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 17, 2007·Developmental and Comparative Immunology·Katrin UtkeUwe Fischer
Nov 27, 2008·Fish & Shellfish Immunology·Rong HuangQiong-Lin Guo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.