Molecular cloning, characterization, and promoter analysis of the mouse Crp2/SmLim gene. Preferential expression of its promoter in the vascular smooth muscle cells of transgenic mice.

The Journal of Biological Chemistry
Shaw-Fang YetM E Lee

Abstract

Several members of the LIM protein family have important roles in development and differentiation. We recently isolated a rat cDNA encoding a new member of this family, CRP2/SmLIM, that contains two LIM domains and is expressed preferentially in vascular smooth muscle cells (VSMC). To study the molecular mechanisms that regulate VSMC-specific transcription of the Crp2/SmLim gene, we cloned the cDNA and gene of mouse Crp2/SmLim. Mouse Crp2/SmLim is a single copy gene of six exons and five introns spanning approximately 20 kilobases of genomic DNA. By 5'-rapid amplification of cDNA ends and S1 nuclease protection assay, we determined that the transcription start site is an A residue 80 base pairs 5' of the translation initiation codon. A TATA-like sequence is located 27 base pairs 5' of the transcription start site, and there are potential cis-acting elements (GATA, Sp1, AP-2, E box, CCAC box, and GArC motif) in the 5'-flanking sequence. In transient transfection assays in rat aortic smooth muscle cells in primary culture, 5 kilobases of the Crp2/SmLim 5'-flanking sequence generated a high level of luciferase reporter gene activity. By deletion analysis and gel mobility shift assay, we found that the region between bases -74 and ...Continue Reading

References

Jul 10, 1992·Cell·A Bird
Dec 1, 1992·The Journal of Cell Biology·I SadlerM C Beckerle
Aug 11, 1991·Nucleic Acids Research·P W LairdA Berns
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S T SmaleD Baltimore
Sep 1, 1990·Genes & Development·E N Olson
Nov 11, 1988·Nucleic Acids Research·J D Hawkins
Apr 8, 1988·Cell·H Cedar
Dec 22, 1986·Nucleic Acids Research·P Bucher, E N Trifonov
Feb 1, 1982·The Journal of Cell Biology·S GuntherM A Gimbrone
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Dec 1, 1995·The Journal of Biological Chemistry·R WeiskirchenM C Beckerle
Jul 1, 1995·Physiological Reviews·G K Owens
Oct 1, 1994·Molecular Biology of the Cell·W F DenetclawR A Steinhardt
Sep 1, 1994·Trends in Genetics : TIG·I Sánchez-García, T H Rabbitts
Jan 28, 1994·Cell·G L Verdine
Jul 12, 1996·Cell·G Felsenfeld
Sep 1, 1996·The Journal of Cell Biology·B E StronachM C Beckerle

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Citations

Jan 16, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Chung-Huang ChenShaw-Fang Yet
Apr 15, 2004·Biochemical and Biophysical Research Communications·Keon MenziesMark B Taubman
Jun 1, 2006·Biochemical and Biophysical Research Communications·Jens HerrmannRalf Weiskirchen
Jul 20, 2002·Mechanisms of Development·Marie Ange BonninDelphine Duprez
May 13, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Meena S Kumar, Gary K Owens
Feb 25, 2006·Endocrinology·Jakomijn HoogendamMarcel Karperien
Jun 7, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Yung-Fu ChangShaw-Fang Yet
Jul 23, 2004·Physiological Reviews·Gary K OwensBrian R Wamhoff
Feb 5, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L C FosterM A Perrella
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Joseph M MianoEmilio Garcia
Jul 19, 2014·Molecular Biology Reports·Chung-Huang ChenShaw-Fang Yet

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