PMID: 2115872Aug 1, 1990Paper

Molecular cloning, characterization, and regulation of a Pseudomonas pickettii PKO1 gene encoding phenol hydroxylase and expression of the gene in Pseudomonas aeruginosa PAO1c

Journal of Bacteriology
J J Kukor, R H Olsen

Abstract

A 26-kilobase BamHI restriction endonuclease DNA fragment was cloned from Pseudomonas pickettii PKO1, a strain isolated from a soil microcosm that had been amended with benzene, toluene, and xylene. This DNA fragment, cloned into vector plasmid pRO1727 and designated pRO1957, allowed Pseudomonas aeruginosa PAO1c to grow on phenol as the sole source of carbon. Physical and functional restriction endonuclease maps have been derived for the cloned DNA fragment. Two DNA fragments carried in trans and derived from subclones of pRO1957 show phenol hydroxylase activity in cell extracts of P. aeruginosa. Deletion and subcloning analyses of these fragments indicated that the gene encoding phenol hydroxylase is positively regulated. Phenol and m-cresol were shown to be inducers of the enzyme. o-Cresol and p-cresol did not induce enzymatic activity but could be metabolized by cells that had been previously exposed to phenol or m-cresol; moreover, the enzyme exhibited a rather broad substrate specificity and was sensitive to thiol-inhibiting reagents. A novel polypeptide with an estimated molecular mass of 80,000 daltons was detected in extracts of phenol-induced cells of P. aeruginosa carrying plasmid pRO1959.

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Citations

Jun 1, 1995·Environmental Health Perspectives·R H OlsenA M Byrne
Jun 26, 2013·Genome Announcements·Sagar M UtturkarSteven D Brown
Sep 18, 2015·The Journal of General and Applied Microbiology·Xiao XiaoXihui Shen
Oct 1, 2013·Ground Water Monitoring & Remediation·Katherine C KeyDora Ogles
Jul 8, 2015·Applied and Environmental Microbiology·P M Martínez-LavanchyH J Heipieper
Oct 1, 2019·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Rushika PatelNasreen S Munshi
Apr 20, 1995·Molecular & General Genetics : MGG·H HerrmannK Hahnke
Jun 1, 1996·Biodegradation·S DyreborgK Broholm
Sep 28, 2007·Journal of Applied Microbiology·M P RyanC C Adley
Sep 1, 1995·Applied and Environmental Microbiology·R R FulthorpeJ M Tiedje
Apr 1, 1995·Applied and Environmental Microbiology·I C Kim, P J Oriel
Jul 1, 1991·Applied and Environmental Microbiology·M S ShieldsP H Pritchard
Jun 1, 1994·Journal of Bacteriology·R H OlsenB Kaphammer
Sep 1, 1994·Applied and Environmental Microbiology·B E HaiglerJ C Spain
May 1, 1996·Applied and Environmental Microbiology·J J Kukor, R H Olsen
Nov 1, 1991·Applied and Environmental Microbiology·B E Haigler, J C Spain
Jul 1, 1991·Archives of Biochemistry and Biophysics·V RadjendiraneC S Vaidyanathan
Jun 1, 2005·Research in Microbiology·Viviana IzzoAlberto Di Donato

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