Molecular cloning, characterization and tissue specificity of the expression of the ovine CSRP2 and CSRP3 genes from Small-tail Han sheep (Ovis aries)

Gene
Guanqing LiuJianmin Wang

Abstract

Two genes, cysteine- and glycine-rich protein 2 (CSRP2) and cysteine- and glycine-rich protein 3 (CSRP3), play important roles in tissue-specific cell growth and development. However, few CSRP2 and CSRP3 genes have been functionally characterized in sheep. In this study, the full-length cDNAs of the CSRP2 and CSRP3 genes were cloned from Small-tail Han sheep by rapid amplification of cDNA ends-PCR. The GenBank accession numbers of the full-length CSRP2 and CSRP3 cDNA sequences are KJ743957 and KJ743958, respectively. The full-length cDNA of ovine CSRP2 was 917bp, with a 582-bp open reading frame encoding 193 amino acids. The complete ovine CSRP3 cDNA was 917bp, with a 585-bp open reading frame encoding 194 amino acids. Alignment and phylogenetic analyses revealed that their amino acid sequences are highly similar to those of other vertebrates, all of which contain two conserved LIM-only domains and a relatively conserved nuclear targeting sequence. To further validate the functions of the two genes, their mRNA expression patterns were evaluated in various Small-tail Han and Dorper sheep tissues using qRT-PCR analyses. CSRP2 was mainly detected in the aorta, whereas CSRP3 was highly concentrated in the heart and the muscle. CSRP...Continue Reading

References

Dec 1, 1995·The Journal of Biological Chemistry·R WeiskirchenM C Beckerle
May 2, 1997·The Journal of Biological Chemistry·R KonratK Bister
Feb 1, 1997·Molecular Biology of the Cell·K L Schmeichel, M C Beckerle
Aug 1, 1997·Molecular and Cellular Biology·Y KongS F Konieczny
Dec 29, 1998·Mechanisms of Development·J M Delalande, P Y Rescan
Dec 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·James R HendersonMary C Beckerle
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 18, 2003·Developmental Cell·David F ChangRobert J Schwartz
Jan 23, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ralf Weiskirchen, Kalle Günther
Oct 2, 2003·Human Molecular Genetics·Sara T WinokurKevin M Flanigan
Jan 27, 2004·FEBS Letters·Markus Grubinger, Mario Gimona
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Julie L Kadrmas, Mary C Beckerle
Aug 12, 2005·American Journal of Physiology. Cell Physiology·Ilona A BarashRichard L Lieber
Aug 1, 2006·Human Molecular Genetics·Despina SanoudouAlan H Beggs
Dec 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·David F ChangRobert J Schwartz
Jan 2, 2010·Circulation Research·Ralph KnöllKenneth R Chien
Nov 19, 2011·Genetics and Molecular Research : GMR·T T PinheiroA Figueira
Apr 3, 2012·Nature Medicine·Wei GuoMichael Gotthardt
May 28, 2014·The FEBS Journal·Elizabeth VafiadakiDespina Sanoudou

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Citations

Oct 13, 2019·Journal of Proteomics·Yongfu LaMingxing Chu

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