Molecular cloning, expression analysis, and appetite regulatory effect of peptide YY in Siberian sturgeon (Acipenser baerii)

Gene
Hu ChenZhiqiong Li

Abstract

Peptide YY (PYY) is an anorectic brain-gut peptide involved in feeding regulation and well characterized in mammals. However, the functional role of PYY in the appetite regulatory of fish is not clear. In this study, we characterized a high conservation of PYY cDNA and found high expression levels of PYY mRNA in the brain and digestive tract of Siberian sturgeon. Then, we examined preprandial (pre- and post-feeding) changes of PYY mRNA expression in the brain that showed a significantly increased in 3h post-feeding, suggesting an anorectic possible function of PYY in Siberian sturgeon. Next, we examined the expression of PYY mRNA during 15 days fasting and refeed after fasting. The SsPYY mRNA expression of unfed fish had a significant 2.4, 1.7, 2.0, 2.2, and 2.1-fold decrease compared to 1-, 3-, 6-, 10- and 15-day ad libitum fed animals, respectively. After refeed, SsPYY mRNA significantly increased 1.9 and 4.1-fold above that of the 15-day fed and unfed fish control group (P<0.01). Furthermore, a single intraperitoneal injection of 10, 100 and 200 ng/g BW SsPYY1-36 caused a reduction in the next feeding and no significant reduction in food intake was observed in fish injected with a 1 ng/g BW. Overall, PYY has a potentially ro...Continue Reading

References

Mar 1, 1989·Endocrinology·G H GreeleyJ C Thompson
Dec 22, 1986·Regulatory Peptides·R EkmanP Panula
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K Tatemoto
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M LundbergB Hamberger
Aug 19, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J M Cerdá-Reverter, D Larhammar
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 9, 2002·Nature·Rachel L BatterhamStephen R Bloom
Nov 5, 2004·Current Opinion in Pharmacology·B M C McGowan, S R Bloom
Nov 13, 2004·Endocrinology·Prasanth K ChelikaniRoger D Reidelberger
May 3, 2005·General and Comparative Endocrinology·H VolkoffR E Peter
Aug 19, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Axel Meyer, Yves Van de Peer
Nov 16, 2005·Gastroenterology·Lukas DegenChristoph Beglinger
Apr 13, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Prasanth K ChelikaniRoger D Reidelberger
Oct 24, 2007·General and Comparative Endocrinology·Görel SundströmDan Larhammar
Apr 18, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Suraj Unniappan, Timothy J Kieffer
Jul 30, 2008·Nutrition·Ryo MorimotoKazuhiro Takahashi
Dec 17, 2008·General and Comparative Endocrinology·Koji MurashitaIvar Rønnestad
Oct 6, 2009·General and Comparative Endocrinology·Ronald Gonzalez, Suraj Unniappan
Aug 23, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Helena FällmarDan Larhammar
Jun 7, 2013·Nature Communications·Daniel L RaboskyMichael E Alfaro

❮ Previous
Next ❯

Citations

Jun 2, 2018·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Chaowei ZhouZonglin Zheng
Mar 5, 2020·Fish Physiology and Biochemistry·Dengyue YuanZhiqiong Li
Mar 16, 2019·Frontiers in Endocrinology·Juan Ignacio BertucciSuraj Unniappan
Apr 25, 2017·The Journal of Poultry Science·Kazuhisa HondaHiroshi Kamisoyama
Apr 1, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Ahmed KewanKazuhisa Honda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain & Food Avoidance

Neurons in the basal forebrain play specific roles in regulating feeding. Here are the latest discoveries pertaining to the basal forebrain and food avoidance.

Anorexia Nervosa

Anorexia nervosa (AN) is a psychiatric condition characterized by severe weight loss and secondary problems associated with malnutrition. Here is the latest research on AN.