Molecular cloning, expression, and characterization of acyltransferase from Pseudomonas protegens

Applied Microbiology and Biotechnology
Nina G SchmidtWolfgang Kroutil

Abstract

The formation of C-C bonds by using CoA independent acyltransferases may have significant impact for novel methods for biotechnology. We report the identification of Pseudomonas strains with CoA-independent acyltransferase activity as well as the heterologous expression of the enzyme in E. coli. The cloning strategies and selected expression studies are discussed. The recombinant acyltransferases were characterized with regard to thermal and storage stability, pH,- and co-solvent tolerance. Moreover, the impact of bivalent metals, inhibitors, and other additives was tested. Careful selection of expression and working conditions led to obtain recombinant acyltransferase form Pseudomonas protegens with up to 11 U mL-1 activity.

References

Apr 14, 2005·Journal of the American Chemical Society·Jihane AchkarJ W Frost
Jul 29, 2006·Natural Product Reports·Inder Pal Singh, Sandip B Bharate
Aug 12, 2008·Bioorganic & Medicinal Chemistry·Murat ArtanSe-Kwon Kim
Feb 3, 2009·Applied and Environmental Microbiology·Jennifer A MoynihanE Fidelma Boyd
Feb 25, 2010·Natural Product Reports·Inder Pal SinghWilliam J Foley
Apr 1, 2009·Nature Chemistry·Ryoji Noyori
Apr 20, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jong-Sup Bae
Jun 7, 2011·Applied Microbiology and Biotechnology·Yujin CaoMo Xian
Nov 22, 2011·Applied Microbiology and Biotechnology·Fang Yang, Yujin Cao
Mar 28, 2012·Bioscience, Biotechnology, and Biochemistry·Asuka HayashiKiyofumi Maruyama
Jul 14, 2012·Bioscience, Biotechnology, and Biochemistry·Hiroki SaitouKiyofumi Maruyama
Sep 14, 2012·The Journal of Organic Chemistry·Ramesh MudududdlaSandip B Bharate
Jan 30, 2013·Applied Microbiology and Biotechnology·Guodong RaoHuimin Zhao
Apr 19, 2013·Nature Chemical Biology·Nicholas J Turner, Elaine O'Reilly
May 26, 2017·Angewandte Chemie·Nina G SchmidtWolfgang Kroutil

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Datasets Mentioned

BETA
BAA-477

Methods Mentioned

BETA
acetylation
acylation
PCR
bioacylation

Software Mentioned

BLAST

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