Molecular cloning, expression and subcellular localization of a BiP homolog from rice endosperm tissue

Plant & Cell Physiology
D G MuenchT W Okita

Abstract

The ER luminal binding protein, BiP, has been linked to prolamine protein body formation in rice. To obtain further information on the possible role of this chaperone in protein body formation we have cloned and sequenced a BiP cDNA homolog from rice endosperm. The rice sequence is very similar to the maize BiP exhibiting 92% nucleotide identity and 96% deduced amino acid sequence identity in the coding region. Substantial amino acid sequence homology exists between rice BiP and BiP homologs from several other plant and animal species including long stretches of conservation through the amino-terminal ATPase domain. Considerable variation, however, is observed within the putative carboxy-terminal peptide-binding domain between the plant and nonplant BiP sequences. A single hand of approximately 2.4 kb was visible when RNA gel blots of total RNA purified from seed tissue were probed with radiolabeled rice BiP cDNA. This band increased in intensity during seed development up to 10 days after flowering, and then decreased gradually until seed maturity. Protein gel blots indicated that BiP polypeptide accumulation parallels that of the prolamine polypeptides throughout seed development. Immunocytochemical analysis demonstrated that...Continue Reading

Citations

Mar 5, 2011·Plant Cell Reports·Amélie SaumonneauMathilde Francin-Allami
Sep 25, 2012·Plant Cell Reports·Mathilde Francin-AllamiYves Popineau
Oct 21, 2010·Functional & Integrative Genomics·Maoyun SheWujun Ma
Dec 25, 2012·Cell Stress & Chaperones·Neelam K SarkarAnil Grover
Mar 6, 1999·Current Opinion in Plant Biology·K Matsuoka, S Y Bednarek
Jun 28, 2013·Journal of Agricultural and Food Chemistry·Mária OszvaldLászló Tamás
Sep 11, 2010·The Journal of Biological Chemistry·Immaculada Llop-TousM Dolors Ludevid
Jan 9, 2009·Journal of Experimental Botany·Yuhi SaitoTakehiro Masumura
Oct 27, 2004·Plant Physiology·Davide MainieriAlessandro Vitale
Mar 20, 2012·BMC Plant Biology·Minu JosephJean-Benoit Peltier
Nov 13, 2010·Bioscience, Biotechnology, and Biochemistry·Masaharu KurodaChikako Mikami
Aug 24, 2013·International Journal of Molecular Sciences·Hyun-Jung KimYoung-Mi Kim
Mar 8, 2014·Annals of Botany·Maria OszvaldPaola Tosi
Mar 29, 2003·The Plant Journal : for Cell and Molecular Biology·N D HaganT J V Higgins
Mar 24, 2011·Plant Signaling & Behavior·Dinesh Gupta, Narendra Tuteja
Mar 13, 2015·Plant Signaling & Behavior·Masaru Ohta, Fumio Takaiwa
Feb 2, 2013·Biochimica Et Biophysica Acta·Julie Hollien

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