Molecular cloning, expression pattern and comparative analysis of chitin synthase gene B in Spodoptera exigua

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
N Senthil KumarWenqing Zhang

Abstract

The chitin synthase (CHS) gene B (4781 bp) of Spodoptera exigua (SeCHSB) was cloned by reverse-transcription PCR (RT-PCR) and 3'/5' RACE from the midgut. SeCHSB contains an open reading frame of 4572 nucleotides, encoding a protein of 1523 amino acids with a predicted molecular mass of approximately 174.6 kDa. Alignment of SeCHSB with class B CHSs of other insects showed a high degree of conservation in the putative catalytic domain region. The structure of the SeCHSB gene was analyzed and was found to be the same as that of Manduca sexta CHSB (MsCHSB), including 23 exons and 22 introns but without alternative exons. Southern blot analysis revealed that SeCHSB was a single copy gene and the presence of only two chitin synthase genes in S. exigua. Further investigation indicated that SeCHSB was specifically expressed in the midgut, and its transcript existed constitutively in the midgut from the 3rd instar larval stage to prepupae and reached highest expression on the 1st day of the fifth instar larval stage. These data suggest that SeCHSB is very important in midgut formation and development. Chitin synthase gene comparisons between different classes of insects using software tools revealed some interesting aspects of the simil...Continue Reading

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Citations

Nov 10, 2012·International Journal of Biological Sciences·Raman BansalAndy P Michel
Jun 12, 2012·Insect Biochemistry and Molecular Biology·Xin ZhangKun Yan Zhu
Jun 28, 2011·European Journal of Cell Biology·Hans Merzendorfer
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May 8, 2018·Pest Management Science·Yanjie ZhaoRongqi Liang
Oct 17, 2018·International Journal of Molecular Sciences·Duan TanCheng Lu
May 24, 2020·Current Pharmaceutical Design·Subbaratnam MuthukrishnanYasuyuki Arakane

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