Molecular cloning, expression pattern of follistatin gene and association analysis with growth traits in bighead carp (Hypophthalmichthys nobilis)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Meixia PangYing Zhou

Abstract

Follistatin (FST) is a single-chain gonadal protein involving in various biological effects. FST plays important roles in not only ovary development but also body growth, whereas myostatin (MSTN) negatively regulates muscle growth. In this study, FST gene in bighead carp (HynFST) was cloned and characterized. A 5797 bp genomic sequence of HynFST, consisting six exons and five introns were cloned. The full-length cDNA of HynFST (2134 bp) has an open reading fragment encoding a polypeptide of 349 amino acids. Sequence comparison and phylogenetic analysis confirmed that FSTs are conserved throughout the vertebrates and HynFST belongs to FST-1 isoform. Nine single nucleotide polymorphisms (SNPs) of the HynFST were identified and three of them (g.2443 T > C, g.2852 T > C and g.5483A > G) were significantly associated with four growth-related traits. The average body weight of those fish with the combined genotype (CC CC GG) was 12.15-22.63% higher than that of triplotype (TT TT AA) in two bighead carp populations. HynFST was expressed in most of the development stages and various tissues with highest level in ovary. The co-expression results for FST and MSTN in brain and muscle of divergent weight groups showed that FST may inhibit ...Continue Reading

References

Feb 16, 1990·Science·T NakamuraH Sugino
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N UenoR Guillemin
Dec 16, 1987·Biochemical and Biophysical Research Communications·D M RobertsonD M de Kretser
Feb 1, 1993·Molecular and Cellular Endocrinology·U MichelJ K Findlay
Apr 1, 1997·Mechanisms of Development·A FainsodM Blum
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·S IemuraN Ueno
Jan 12, 1999·Developmental Biology·H BauerM Hammerschmidt
Mar 30, 2001·The Journal of Biological Chemistry·Y SidisH T Keutmann
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·S J Lee, A C McPherron
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 28, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Steven L KleinPaul Richardson
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert L StrausbergUNKNOWN Mammalian Gene Collection Program Team
Dec 25, 2002·Human Reproduction Update·D M de KretserD J Phillips
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Motonobu NakamuraRalf Paus
Mar 6, 2003·Genome Research·John S TaylorYves Van de Peer
Apr 18, 2003·Endocrine Reviews·Ernesto CanalisElisabetta Gazzerro
Jul 31, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Shyr-Yeu LinDavid M de Kretser
Oct 18, 2003·Molecular Endocrinology·Henry T KeutmannYisrael Sidis
Sep 16, 2005·Molecular Biology and Evolution·Karen D CrowGünter P Wagner
Aug 8, 2006·Developmental Biology·Sophie Dal-PraChristine Thisse
Sep 26, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Masashi NakataniKunihiro Tsuchida
Dec 13, 2007·Development Genes and Evolution·Daniel J Macqueen, Ian A Johnston
Jan 2, 2008·Molecular Biology Reports·Bruria FunkensteinTal Skopal
Feb 15, 2011·Science China. Life Sciences·Xi LiJiangYan He
Jul 10, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jianbin NiCaihuan Ke
Dec 4, 2012·International Journal of Molecular Sciences·Yanhong SunJingou Tong
Dec 24, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiu FengJingou Tong
Jun 30, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shilei LiQingzhi Wang
Sep 21, 2016·International Journal of Molecular Sciences·Xinhua WangJingou Tong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.