Molecular cloning, functional characterization and expression of potato (Solanum tuberosum) 1-deoxy-d-xylulose 5-phosphate synthase 1 (StDXS1) in response to Phytophthora infestans

Plant Science : an International Journal of Experimental Plant Biology
Maria Antonia HenriquezFouad Daayf

Abstract

1-Deoxy-D-xylulose 5-phosphate synthase (DXS) catalyzes the initial step of the plastidial 2C-methyl-D-erythritol-4-phosphate (DOXP-MEP) pathway involved in isoprenoid biosynthesis. In this study, we cloned the complete cDNA of potato DXS gene that was designated StDXS1. StDXS1 cDNA encodes for 719 amino acid residues, with MW of 77.8 kDa, and is present in one copy in the potato genome. Phylogenetic analysis and protein sequence alignments assigned StDXS1 to a group with DXS homologues from closely related species and exhibited homodomain identity with known DXS proteins from other plant species. Late blight symptoms occurred in parallel with a reduction in StDXS1 transcript levels, which may be associated with the levels of isoprenoids that contribute to plant protection against pathogens. Subcellular localization indicated that StDXS1 targets the chloroplasts where isoprenoids are synthesized. Arabidopsis expressing StDXS1 showed a higher accumulation of carotenoids and chlorophyll as compared to wild type controls. Lower levels of ABA and GA were detected in the transgenic DXS lines as compared to control plants, which reflected on higher germination rates of the transgenic DXS lines. No changes were detected in JA or SA co...Continue Reading

References

Jul 1, 1995·The Plant Cell·D J McGarvey, R Croteau
May 1, 1996·The Plant Journal : for Cell and Molecular Biology·M A MandelP León
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·L M LoisA Boronat
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·B M LangeR Croteau
Jul 19, 2001·Nature·R A Dixon
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 30, 2003·Biochemical Society Transactions·W N HunterL E Kemp
Jun 16, 2004·Cellular and Molecular Life Sciences : CMLS·W EisenreichF Rohdich
Dec 21, 2004·Nature Reviews. Microbiology·Howard S Judelson, Flavio A Blanco
Sep 1, 2005·Biotechnology Letters·Bo-Ra KimYung-Jin Chang
Apr 7, 2009·Plant Physiology·Ratnakar Vallabhaneni, Eleanore T Wurtzel
Jul 9, 2009·Journal of Experimental Botany·Elizabeth CordobaPatricia León
Jul 15, 2009·The Plant Journal : for Cell and Molecular Biology·Antía Rodríguez-VillalónManuel Rodríguez-Concepción
Jan 5, 2011·Journal of Experimental Botany·Elizabeth CordobaPatricia León
Jul 14, 2012·Journal of Experimental Botany·Bianyun YuAbdelali Hannoufa

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Citations

Feb 2, 2018·Frontiers in Plant Science·Lorenza Dalla CostaM Stella Grando
Nov 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huabei ZhangLuqi Huang
Apr 3, 2019·Molecular Plant-microbe Interactions : MPMI·Michael G BeckerMark F Belmonte
Apr 17, 2019·International Journal of Molecular Sciences·Hui WeiQiang Zhuge

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