PMID: 9555010May 23, 1998Paper

Molecular cloning, functional expression and tissue distribution of rat acyl-coenzyme A:cholesterol acyltransferase

Biochimica Et Biophysica Acta
H MatsudaS Horiuchi

Abstract

Acyl-coenzyme A:cholesterol acyltransferase (ACAT) is an enzyme catalyzing the intracellular formation of cholesteryl esters from free cholesterol and fatty acyl-CoA. In the present study, we cloned rat ACAT cDNA and determined its tissue distribution. Rat ACAT cDNA, having a coding region of 1635 bp with its deduced protein sequence of 545 amino acids and two typical motifs such as signature sequences and leucine heptad motif, showed 83, 92 and 90% identity with human, mouse, and hamster ACAT, respectively. Expression of rat ACAT cDNA in A293 cells and CHO cells resulted in a 3.0 to 3.5-fold increase in the enzyme activity. Among twelve tissues examined, ACAT activity was highest in adrenal followed by liver and intestine while that of aorta was extremely low. The mRNA level was also the highest in adrenal among four tissues examined. However, in contrast to its high ACAT activity, the liver mRNA level was extremely low (adrenal > intestine > aorta > liver). Consistent with mRNA levels, immunohistochemical analyses with a specific ACAT antibody detected significant ACAT signals in adrenal and intestine but a negligible signal in liver. These results indicate that adrenal most abundantly expresses ACAT in rat. Furthermore, rat ...Continue Reading

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Citations

Jun 9, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Maria R Tosi, Vitaliano Tugnoli
Aug 2, 2003·Clinical and Experimental Pharmacology & Physiology·Hiroshi Ogawa, Satoshi Mochizuki
Jul 20, 2012·Journal of Lipid Research·Chang Xie 谢畅Bao-Liang Song 宋保亮
Mar 1, 2014·International Journal of Molecular Sciences·Dong-Feng WuWu-Xian Chen
Mar 7, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Jihei NishimuraYukinori Amano
Oct 9, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·A MiyazakiT Y Chang
Jul 10, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Masaharu HoriSeikoh Horiuchi
Mar 26, 1998·Biochemical and Biophysical Research Communications·T KawasakiS Horiuchi
Aug 18, 1999·Bioorganic & Medicinal Chemistry Letters·N MatsuyamaK Mizuno

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