PMID: 8603840Feb 1, 1996Paper

Molecular cloning, membrane topology, and localization of bovine rom-1 in rod and cone photoreceptor cells

Investigative Ophthalmology & Visual Science
Orson L Moritz, R S Molday

Abstract

To characterize the molecular properties, cellular distribution, and subcellular distribution of bovine rom-1 and its interaction with peripherin/rds in photoreceptor cells as an important step toward elucidating the role of rom-1 in photoreceptor outer segment structure-function relationships and in inherited retinal degenerative disorders. Bovine rom-1 cDNA, including a portion of the promoter region, was cloned, sequenced, and heterologously expressed in CHO-K1 and COS-1 cultured mammalian cells. Monoclonal and polyclonal antibodies to specific regions of bovine rom-1 were generated and used with biochemical and immunocytochemical techniques to study the membrane topology, subcellular distribution, and interaction of rom-1 with peripherin/rds. The primary structure of bovine rom-1 is highly homologous to that of human and mouse rom-1. Proteolytic digestion studies and immunolabeling studies of rom-1 in rod outer segment membranes indicate that the C-terminus of rom-1 is localized to the cytoplasmic side and that a large segment is localized to the lumen side of the disc membrane. Post-embedding and pre-embedding immunogold labeling studies for electron microscopy show that rom-1 is localized to the rim region of bovine rod o...Continue Reading

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