Abstract
An alpha-amylase gene of Bacillus subtilis (natto) IAM1212 was cloned in a lambda EMBL3 bacteriophage vector, and the nucleotide sequence was determined. An open reading frame encoding the alpha-amylase (AMY1212) consists of 1,431 base pairs and contains 477 amino acid residues, which is the same in size as the alpha-amylase (AMY2633) of B. subtilis 2633, an alpha-amylase-hyperproducing strain, and smaller than that of B. subtilis 168, Marburg strain. The amino acid sequence of AMY1212 is different from that of AMY2633 at five residues. Enzymatic properties of these two alpha-amylases were examined by introducing the cloned genes into an alpha-amylase-deficient strain, B. subtilis M15. It was revealed that products of soluble starch hydrolyzed by AMY1212 are maltose and maltotriose, while those of AMY2633 are glucose and maltose. From the detailed analyses with oligosaccharides as substrates, it was concluded that the difference in hydrolysis products of the two similar alpha-amylases should be ascribed to the different activity hydrolyzing low-molecular-weight substrates, especially maltotriose; AMY1212 slowly hydrolyzes maltotetraose and cannot hydrolyze maltotriose, while AMY2633 efficiently hydrolyzes maltotetraose and malt...Continue Reading
References
May 1, 1979·Molecular Immunology·A M PoverennyE D Sverdlov
Nov 1, 1985·Journal of Biochemistry·T YuukiS Udaka
Jul 25, 1988·Nucleic Acids Research·M Emori, B Maruo
Jul 1, 1989·Journal of Bacteriology·M J Weickert, G H Chambliss
Sep 1, 1987·Journal of Bacteriology·K KimuraK Yamane
Mar 1, 1986·Journal of Bacteriology·W L Nicholson, G H Chambliss
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Aug 1, 1974·Journal of Bacteriology·K YamaguchiB Maruo
Sep 13, 1974·Biochimica Et Biophysica Acta·H MatsuzakiB Maruo
Jan 25, 1973·Biochimica Et Biophysica Acta·K YamaneB Maruo
Apr 12, 1973·Biochimica Et Biophysica Acta·K HiromiS Ono
Oct 1, 1967·Journal of Bacteriology·N E Welker, L L Campbell
Apr 14, 1970·Biochemistry·J A ThomaJ Spradlin
Aug 15, 1970·Nature·U K Laemmli
Dec 1, 1984·Journal of Biochemistry·K YamaneA Nakayama
Jan 25, 1983·Nucleic Acids Research·M YangD Henner
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J R Mielenz
Nov 15, 1983·Journal of Molecular Biology·A M FrischaufN Murray
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Oct 1, 1983·Journal of Bacteriology·H YamazakiK Yamane
Citations
Jul 1, 1996·European Journal of Biochemistry·B Boeck, R Schinzel
Oct 4, 2005·The Biochemical Journal·Mamdouh Ben AliSamir Bejar
May 23, 2002·FEMS Microbiology Letters·Fabrice SabathéPhilippe Soucaille
Nov 15, 2011·Marine Biotechnology·Yang LiuHui Peng
Jul 1, 1991·Journal of Bacteriology·E RumbakD R Woods