Molecular cloning of 4-coumarate:coenzyme A ligase in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood

Plant Physiology
X H Zhang, V L Chiang

Abstract

Two genomic sequences encoding 4-coumarate:coenzyme A ligase (4CL; EC 6.2.1.12) in loblolly pine (Pinus taeda L.) were cloned. Both sequences contained three introns and four exons with identical coding sequences predicting 537 amino acids. Two of the three introns in these two clones were different both in sequence and in length. Sequences of both 4CL clones were found in all nine megagametophyte DNAs tested, providing genetic evidence that these two 4CL genomic sequences are nonallelic genes. Our analyses suggest that there are at least two distinct, intron-containing 4CL genes, at least one of which is transcribed into 4CL mRNA in developing xylem tissue of loblolly pine. The levels of 4CL gene transcription in xylem were influenced by compressional stress, resulting in an elevated 4CL enzyme activity with 4-coumaric acid. 4CL enzyme activity with ferulic acid remained unchanged, whereas with caffeic acid it was significantly inhibited. Exogenously applied trans-cinnamic acid in the protein extracts from normal wood xylem caused inhibition of 4CL activity toward caffeic acid similar to that under compressional stress. The implications of this cinnamic acid-modulated effect on 4CL enzyme activities toward different substrates...Continue Reading

References

Mar 17, 1975·European Journal of Biochemistry·K H Knobloch, K Hahlbrock
Oct 25, 1990·Nucleic Acids Research·Y ZhaoS K Dube
Mar 1, 1974·European Journal of Biochemistry·G G Gross, M H Zenk

❮ Previous
Next ❯

Citations

Jan 26, 2012·Biochemical Genetics·Ha Van HuanSiliang Zhang
Apr 6, 2004·Molecular Phylogenetics and Evolution·Xiao-Xin Wei, Xiao-Quan Wang
May 8, 2013·Journal of Agricultural and Food Chemistry·Ting-Ting YouFeng Xu
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·J EhltingE Kombrink
Feb 22, 2002·European Journal of Biochemistry·Christian LindermayrJürgen Ebel
Apr 26, 2000·Molecular Biology and Evolution·X Q WangT Sang
Oct 31, 2008·Plant Physiology·Armin WagnerJohn Ralph
Mar 7, 2003·Applied and Environmental Microbiology·Xue PengShigeaki Harayama
Jun 4, 2005·Applied and Environmental Microbiology·Hanxiao JiangJohn A Morgan
Nov 2, 2011·Genome Génome / Conseil National De Recherches Canada·Jiangfeng YouZhenming Yang
Nov 18, 2006·Genetics·Santiago C González-MartínezDavid B Neale
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·L LiV L Chiang
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·W J HuV L Chiang
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·K OsakabeV L Chiang
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·I AllonaR W Whetten
Feb 7, 2017·International Journal of Molecular Sciences·Juan Domínguez-RoblesAlejandro Rodríguez
Jul 20, 2005·Plant Molecular Biology·Freydoun GarabagiJudith Strommer
Oct 13, 2016·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Huynh Thi Thu HueLe Thi Thu Hien

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.