PMID: 9421930Jan 9, 1998Paper

Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)

Planta
J Derek BewleyG B Fincher

Abstract

Mannose-containing polysaccharides are widely distributed in cell walls of higher plants. During endosperm mobilization in germinated tomato seeds (1-->4)-beta-mannan endohydrolases (EC 3.2.1.78) participate in the enzymic depolymerization of these cell wall polysaccharides. A cDNA encoding a (1-->4)-beta-mannanase from the endosperm of germinated tomato (Lycopersicon esculentum Mill.) seeds has been isolated and characterized. The amino acid sequence deduced from the 5'-region of the cDNA exactly matches the sequence of the 65 NH2-terminal amino acids determined directly from the purified enzyme. The mature enzyme consists of 346 amino acid residues, it has a calculated M(r) of 38,950 and an isoelectric point of 5.3. Overall, the enzyme exhibits only 28-30% sequence identity with fungal (1-->4)-beta-mannanases, but more highly conserved regions, which may represent catalytic and substrate-binding domains, can be identified. Based on classification of the tomato (1-->4)-beta-mannanase as a member of the family 5 group of glycosyl hydrolases, Glu-148 and Glu-265 would be expected to be the catalytic acid and the catalytic nucleophile, respectively. Southern hybridization analyses indicate that the enzyme is derived from a family...Continue Reading

Citations

Aug 10, 2006·Functional & Integrative Genomics·Joshua S YuanFeng Chen
May 4, 2012·Journal of Experimental Botany·María del Carmen Rodríguez-GacioAngel J Matilla
Feb 21, 2004·Plant Physiology·Sergei A FilichkinHiroyuki Nonogaki
Feb 2, 2008·Chemical & Pharmaceutical Bulletin·Jong Keun SonMi Hee Woo
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Dec 14, 2011·Physiologia Plantarum·Sue Ellen Ester QueirozHenk W M Hilhorst
Oct 7, 2006·Plant Physiology and Biochemistry : PPB·Z Minic, L Jouanin
Jun 22, 2006·Journal of Biochemistry·Takeshi IshimizuSumihiro Hase
Dec 20, 2012·Molekuliarnaia genetika, mikrobiologiia i virusologiia·Jun Li HuangGui Xue Wang
Jul 13, 2004·The Journal of Biological Chemistry·Takeshi IshimizuSumihiro Hase

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