Molecular cloning of a cDNA encoding a crustacean hyperglycemic hormone from eyestalk ganglia of the blue crab, Callinectes sapidus

General and Comparative Endocrinology
Cheol Young ChoiR Douglas Watson

Abstract

Crustacean hyperglycemic hormone (CHH), a polypeptide with multiple physiological effects, was first identified in the X-organ/sinus gland neurosecretory system of the eyestalks. In studies reported here, we used a PCR-based cloning strategy (RT-PCR followed by 5'- and 3'-RACE) to clone from blue crab (Callinectes sapidus) eyestalk ganglia a cDNA (CsCHH-1) encoding a putative CHH preprohormone. Sequence analysis revealed the preprohormone included all structural features previously reported for CHH preprohormones: a signal peptide, a CHH precursor-related peptide (CPRP), the CHH polypeptide, and a C-terminal basic processing site. Further, the deduced amino acid sequence of the mature polypeptide included all signature domains previously reported for CHH. The primary structure of blue crab CHH is most closely related to CHH from other brachyurans. RT-PCR revealed the CsCHH-1 transcript was present in eyestalk ganglia, but was undetectable in other tissues tested. A transcript encoding a similar CHH-like preprohormone was detected in thoracic ganglion, ventral nerve cord, and brain, but was not detected in eyestalk ganglia.

References

Jun 11, 1986·Nucleic Acids Research·G von Heijne
Dec 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D P De Kleijn, F Van Herp
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J S ChungS G Webster
Oct 6, 2000·General and Comparative Endocrinology·C Spanings-PierrotG Charmantier
May 10, 2005·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Deug-Woo Han, R Douglas Watson

❮ Previous
Next ❯

Citations

Oct 30, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Michael J StewartPrasert Sobhon
May 9, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ye-Qing QianWei-Jun Yang
Mar 7, 2008·General and Comparative Endocrinology·Andrew E ChristiePatsy S Dickinson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.