Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa

FEBS Letters
K ToriyamaM Watanabe

Abstract

A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.

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Citations

Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·P. Schurmann, J.-P. Jacquot
Dec 12, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Masao WatanabeGo Suzuki
May 20, 2005·Journal of Plant Physiology·Kenneth J McNeil, Alan G Smith
Sep 21, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Díaz-PeralesG Salcedo
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·R Sánchez-MongeG Salcedo
Feb 22, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S BérotJ Guéguen
Jun 28, 2001·Plant & Cell Physiology·M WatanabeK Hinata
Jan 15, 2003·The Journal of Biological Chemistry·Jean-Luc PonsMarc-André Delsuc

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