Abstract
A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.
References
Apr 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P J FellJ Brostoff
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M B SinghJ McCluskey
Aug 2, 1991·Science·R ValentaO Scheiner
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 1, 1995·Planta·S J HiscockH G Dickinson
Sep 1, 1995·International Archives of Allergy and Immunology·B P SinghS V Gangal
Jan 1, 1995·Planta·S J CrossleyH G Dickinson
Dec 1, 1994·Plant & Cell Physiology·M WatanabeK Hinata
Jan 1, 1995·Allergy·Y TaniguchiT Matuhasi
Dec 1, 1995·Plant Molecular Biology·K ToriyamaM B Singh
May 1, 1996·The Plant Journal : for Cell and Molecular Biology·J H Ross, D J Murphy
Aug 1, 1996·The Plant Journal : for Cell and Molecular Biology·B S StanchevR R Croy
Jun 1, 1996·Plant Physiology·C O LimM J Cho
Sep 1, 1996·The Plant Cell·M S BowerD R Goring
Feb 3, 1997·FEBS Letters·C SuphiogluR B Knox
May 1, 1997·Plant Molecular Biology·R K RuiterG J Wullems
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J DoughtyH Dickinson
Dec 1, 1990·The Plant Cell·A. M. KoltunowR. B. Goldberg
Feb 1, 1992·Planta·D E EvansR B Knox
Citations
Jan 7, 2006·Plant Cell Reports·Jong-In ParkMasao Watanabe
Oct 26, 1999·Immunology Today·D J Murphy
Dec 18, 2001·Plant Physiology·D M Paxson-SowdersC A Makaroff
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·P. Schurmann, J.-P. Jacquot
Apr 27, 2010·PloS One·Katinka A Vigh-ConradDaphne Preuss
Dec 12, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Masao WatanabeGo Suzuki
May 20, 2005·Journal of Plant Physiology·Kenneth J McNeil, Alan G Smith
Sep 21, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Díaz-PeralesG Salcedo
Aug 6, 2013·Molecular Nutrition & Food Research·Eva VejvarGabriele Gadermaier
Mar 9, 1999·The Journal of Allergy and Clinical Immunology·R Sánchez-MongeG Salcedo
Apr 23, 2008·Plant Biology·L HuangY Ye
Mar 21, 2015·Molecular Immunology·Ivana GiangriecoAdriano Mari
Feb 22, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S BérotJ Guéguen
Jun 28, 2001·Plant & Cell Physiology·M WatanabeK Hinata
Jan 15, 2003·The Journal of Biological Chemistry·Jean-Luc PonsMarc-André Delsuc
Apr 12, 2002·FEBS Letters·Makoto EndoMasao Watanabe
Nov 24, 2007·Molecular Biology Reports·Aimei TianWanzhi Ye
Aug 21, 2007·Peptides·Peng LinT B Ng