PMID: 7546290Oct 1, 1995Paper

Molecular cloning of a cDNA encoding human spermine synthase

DNA and Cell Biology
V P KorhonenJ Jänne

Abstract

We have isolated and sequenced cDNA clones that encode human spermine synthase (EC 2.5.1.22). The total length of the sequenced cDNA was 1,612 nucleotides, containing an open reading frame encoding a polypeptide chain of 368 amino acids. All of the previously sequenced peptide fragments of human and bovine spermine synthase proteins could be located within the coding region derived from the cDNA. An unusual sequence of AATTAA apparently signaled the initiation of polyadenylation. Sequence comparisons between human spermine synthase and spermidine synthases from bacterial and mammalian sources revealed a nearly complete lack of similarity between the primary structures of these two enzymes catalyzing almost identical reactions. A modest similarity found was restricted to a relatively short peptide domain apparently involved in the binding of decarboxylated S-adenosylmethionine, the common substrate for both enzymes. The apparent lack of an overall similarity may indicate that spermine synthase, the enzyme found only in eukaryotes, and spermidine synthase with more universal distribution, although functionally closely related, have evolved separately.

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Citations

Jun 23, 2001·Molecular and Biochemical Parasitology·S C RobertsB Ullman
Feb 26, 2013·Expert Reviews in Molecular Medicine·Shannon L NowotarskiRobert A Casero
Apr 28, 2007·Nature Reviews. Drug Discovery·Robert A Casero, Laurence J Marton
May 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Erin K WillertMargaret A Phillips
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A M WallaceC C MacDonald
Oct 21, 2000·Journal of Molecular and Cellular Cardiology·A N LopatinA E Pegg
Jul 30, 2016·The Biochemical Journal·Anthony J Michael
Aug 31, 2019·The Biochemical Journal·Bin LiAnthony J Michael
Sep 30, 2016·The Biochemical Journal·Tracy R Murray-StewartRobert A Casero

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