PMID: 2509432Nov 1, 1989Paper

Molecular cloning of a cell wall exo-beta-1,3-glucanase from Saccharomyces cerevisiae

Journal of Bacteriology
F Klebl, W Tanner

Abstract

A major protein of Saccharomyces cerevisiae cell walls is a 29-kilodalton glycoprotein which shows lectinlike binding to beta-1,3-glucan and chitin. It was solubilized by heating isolated cell walls at 90 degrees C and purified to homogeneity by running two high-pressure liquid chromatography columns. With the sequence information of the N terminus and seven peptides, two oligonucleotides were synthesized and the gene was cloned. Its sequence is similar to those of two plant beta-glucanases, and the protein was shown to possess beta-1,3-exoglucanase activity with laminarin as substrate. Haploid yeast cells contained one copy of the gene (BGL2). Gene disruption did not result in a phenotype.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 1, 1979·Microbiological Reviews·V Farkas
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M J Kuranda, P W Robbins
Apr 27, 1987·Biochimica Et Biophysica Acta·W Tanner, L Lehle
Jan 29, 1985·Biochemistry·J Frevert, C E Ballou
Jun 1, 1971·Bacteriological Reviews·O Necas
Sep 15, 1973·Biochimica Et Biophysica Acta·V FarkasS Bauer
Apr 24, 1982·Nucleic Acids Research·M J DobsonL A Fothergill
May 5, 1983·Journal of Molecular Biology·J A Gough, N E Murray
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S D EmrJ Thorner
Apr 29, 1982·Biochemical and Biophysical Research Communications·F del ReyC Nombela
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G B FincherK K Thomsen
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H ShinshiF Meins

❮ Previous
Next ❯

Citations

May 1, 1991·Yeast·J G De Nobel, J A Barnett
Oct 25, 1996·The Journal of Biological Chemistry·R P HartlandJ P Latgé
Dec 6, 2001·European Journal of Biochemistry·A A KulminskayaK N Neustroev
Jan 11, 2011·Applied and Environmental Microbiology·Sabine SchmidtStefan Martens
Sep 10, 2013·Biochemistry. Biokhimii︠a︡·V V SovaT N Zvyagintseva
Aug 8, 2015·Acta Crystallographica. Section D, Biological Crystallography·Zhen QinShiwang Wu
Apr 7, 1995·The Journal of Biological Chemistry·L ChenP B Høj
Feb 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G A Stewart
Jul 17, 2004·Microbiology·David J Adams
May 5, 2006·Molecular Biology of the Cell·Gertien J SmitsFrans M Klis
Feb 25, 2006·Yeast·Frans M KlisPiet W J De Groot
May 18, 2019·The Journal of Infectious Diseases·Geetha KuttyJoseph A Kovacs
Nov 22, 1991·Proceedings. Biological Sciences·R P HartlandP A Sullivan
Jul 1, 1994·Yeast·F M Klis
Dec 13, 2006·Molecular Plant-microbe Interactions : MPMI·Harold J G MeijerPiet W J de Groot
Nov 23, 2012·FEMS Microbiology Letters·Alice G SorgoFrans M Klis
Aug 21, 2013·Current Genetics·R Teparić, Vladimir Mrsa
Mar 30, 2017·International Journal of Molecular Sciences·Jaime Moreno-GarcíaTeresa García-Martínez
Mar 4, 1998·Yeast·P Van RensburgI S Pretorius
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius

❮ Previous
Next ❯

Related Concepts

Related Feeds