Molecular cloning of a gene selectively induced by gamma interferon from human macrophage cell line U937.

Molecular and Cellular Biology
X D FanB R Bloom

Abstract

A cDNA clone encoding a gamma interferon (IFN-gamma)-inducible mRNA in human cells of the macrophage lineage was isolated and characterized. The corresponding gene, gamma.1, was selectively induced by IFN-gamma, responding a hundredfold better to IFN-gamma than to IFN-alpha. The induction was rapid and transient, with maximal mRNA accumulation at about 3 h and decline to the basal level after 48 h. Transcriptional activation could be detected as early as 5 min after IFN-gamma stimulation and accounted entirely for the mRNA accumulation. The induction of gamma.1 by IFN-gamma was cell-type restricted, being seen only in macrophages and endothelial cells. In addition, phorbol ester-induced differentiation of promyelocytic HL-60 cells and promonocytic THP-1 cells rendered the gamma.1 gene inducible by IFN-gamma. The 1.0-kilobase gamma.1 cDNA sequence encoded a small predicted polypeptide of 38 amino acids and had a conserved sequence associated with rapidly turning over mRNAs. In vitro translation of the gamma.1 transcript yielded a 4,000-dalton polypeptide.

References

May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G RoveraC Damsky
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P E NewburgerS H Orkin
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·X D FanB R Bloom
Jan 1, 1964·Biochemical and Biophysical Research Communications·J Taylor
Sep 1, 1968·Cancer·J A LevyV Defendi
Dec 24, 1981·Nature·A A Branca, C Baglioni
Jan 1, 1983·Advances in Immunology·R M Friedman, S N Vogel
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A C LarnerJ E Darnell
Jul 1, 1983·The Journal of Experimental Medicine·H W MurrayD M Cartelli
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada

❮ Previous
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Citations

Jan 1, 1996·Cancer Investigation·D V Kalvakolanu, E C Borden
Aug 15, 1991·European Journal of Biochemistry·B R Williams
Jun 11, 2004·American Journal of Physiology. Cell Physiology·Tiffany A WallacePeter P Sayeski
Jun 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S Y ChonS L Gupta
Feb 1, 1995·Pharmacology & Therapeutics·M David

❮ Previous
Next ❯

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