PMID: 8962057Dec 10, 1996Paper

Molecular cloning of a high-affinity receptor for the growth factor-like lipid mediator lysophosphatidic acid from Xenopus oocytes

Proceedings of the National Academy of Sciences of the United States of America
Z GuoG Tigyi

Abstract

Lysophosphatidic acid (1-acyl-2-lyso-snglycero-3-phosphate, LPA) is a multifunctional lipid mediator found in a variety of organisms that span the phylogenetic tree from humans to plants. Although its physiological function is not clearly understood, LPA is a potent regulator of mammalian cell proliferation; it is one of the major mitogens found in blood serum. In Xenopus laevis oocytes, LPA elicits oscillatory Cl- currents. This current, like other effects of LPA, is consistent with a plasma membrane receptor-mediated activation of G protein-linked signal transduction pathways. Herein we report the identification of a complementary DNA from Xenopus that encodes a functional high-affinity LPA receptor. The predicted structure of this protein of 372 amino acids contains features common to members of the seven transmembrane receptor superfamily with a predicted extracellular amino and intracellular carboxyl terminus. An antisense oligonucleotide derived from the first 5-11 predicted amino acids, selectively inhibited the expression of the endogenous high-affinity LPA receptors in Xenopus oocytes, whereas the same oligonucleotide did not affect the low-affinity LPA receptor. Expression of the full-length cRNA in oocytes led to an ...Continue Reading

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G TigyiR Miledi
Jun 2, 1995·The Journal of Biological Chemistry·W H Moolenaar
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·G TigyiR Miledi
Jun 15, 1993·Biochemical and Biophysical Research Communications·F ImamuraH Akedo

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Citations

Mar 8, 2000·Cell Motility and the Cytoskeleton·T J ManningH Sontheimer
Sep 30, 1998·Journal of Neuroscience Research·F W HoltsbergS M Steiner
Dec 10, 1999·Journal of Neurobiology·W L ZhouM Yamashita
Feb 19, 2000·Biochemical and Biophysical Research Communications·Y YamazakiH Ohta
Jul 13, 2000·Biochemical and Biophysical Research Communications·L R FitzgeraldP Tsui
Nov 27, 1999·Life Sciences·T KobayashiK Murakami-Murofushi
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·J R EricksonG B Mills
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Jun 19, 2002·Biochimica Et Biophysica Acta·Denis EnglishJoe G N Garcia
Jun 19, 2002·Biochimica Et Biophysica Acta·Xianjun FangGordon B Mills
Jun 19, 2002·Biochimica Et Biophysica Acta·Kazuaki YokoyamaRobert Bittman
Mar 5, 1999·Biochimica Et Biophysica Acta·T A GarrettC R Raetz
Jul 30, 1999·Biochimica Et Biophysica Acta·S A Rudge, J Engebrecht
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Aug 5, 1999·The Biochemical Journal·K MasudaT Shimizu
Mar 21, 2001·Biotechnology & Genetic Engineering Reviews·D J Durzan
Jan 20, 2012·Biology Letters·K C KingM Neiman

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