PMID: 9545573Apr 18, 1998Paper

Molecular cloning of a human cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein

Biochimica Et Biophysica Acta
R KatashimaM Itakura

Abstract

A human cDNA encoding 41-kDa phosphoribosylpyrophosphate (PRPP) synthetase (PRS)-associated protein (PAP41) was cloned from two expressed sequence tag (EST) clones having the nucleotide similarity of 61.5 and 70.0% to human PAP39 cDNA. The predicted open reading frame of 1107 base pairs (bp) has the nucleotide identity of 91.8% to rat PAP41 and encodes a protein of 369 amino acids with a calculated molecular weight (MW) of 40,925. The deduced amino acid sequence exhibits the 98.9% identity to rat PAP41 and 72.2, 50.6, and 50.0% identity with human PAP39, PRS I, and PRS II, respectively, but lacks the PRPP binding site. Southern blot analysis suggested that the PAP41 gene exists as a single copy in the human genome. The single PAP41 mRNA of about 2.1 kb was shown to be present in five human cell lines by Northern blot analysis.

References

Sep 12, 1989·Nucleic Acids Research·V Barbu, F Dautry
Jan 1, 1995·Advances in Enzyme Regulation·M TatibanaI Ahmad

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Citations

Jul 7, 2011·PloS One·Tsutomu OkaHitoshi Okazawa
Sep 17, 2009·Biochemical Society Transactions·Anna KleineidamMichael Schweizer

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