Abstract
Development of pili on cells of Arthrobacter photogonimos is induced by photo-oxidative conditions. The nucleotide sequence was determined of a light-inducible gene (lipA) that encodes the precursor of a light-inducible pilin (designated LIP), a polypeptide of 212 amino acids. The N-terminal leader peptide includes a typical signal sequence with a consensus cleavage site for signal peptidase 1 after residue 28, which should generate N-terminal arginine. However, the next amino acid, alanine, is the N-terminal residue of the mature protein. The abundance of charged amino acids (27% of total), a calculated pI of 9.98, and recovery of mostly monomers when cells were washed with 1 M NaCl suggest that electrostatic interactions play a dominant role in association of LIP, a novel mechanism for assembly of pili.
References
Oct 1, 1978·Journal of Bacteriology·J K Hoober
Nov 1, 1991·Infection and Immunity·K DenichP D O'Hanley
Dec 1, 1991·Gene·A DazaJ A Gil
Jun 15, 1991·Gene·B J EikmannsH Sahm
May 1, 1990·Journal of Bacteriology·M K Yeung, J O Cisar
May 1, 1990·The Journal of Membrane Biology·G von Heijne
Apr 15, 1987·The Biochemical Journal·A N RobertsS Brenner
Jan 1, 1972·Antonie van Leeuwenhoek·W H Crombach
Jan 1, 1972·Infection and Immunity·N Kumazawa, R Yanagawa
Jan 1, 1981·Annual Review of Microbiology·L GoldG Stormo
Apr 1, 1983·Photochemistry and Photobiology·J K Hoober, J Franzi
Jun 1, 1980·Archives of Biochemistry and Biophysics·J K HooberL P D'Angelo
Sep 25, 1995·Nucleic Acids Research·W R Bourn, B Babb
Aug 1, 1995·Applied and Environmental Microbiology·T Dairi, Y Asano
Nov 1, 1994·Molecular Microbiology·H CaustonC F Higgins
Jun 11, 1994·Nucleic Acids Research·I Mahadevan, I Ghosh
Oct 1, 1996·FEMS Microbiology Reviews·O Mol, B Oudega
Jul 1, 1992·Plant Physiology·J K Hoober, M J Hughes