Molecular cloning of a new secreted sulfated mucin-like protein with a C-type lectin domain that is expressed in lymphoblastic cells.

The Journal of Biological Chemistry
S BannwarthJ C Lefebvre

Abstract

We have previously demonstrated hyposialylation of the two major CD45 and leukosialin (CD43) molecules at the surface of latently human immunodeficiency virus type 1-infected CEM T cells (CEMLAI/NP), (Lefebvre, J. C., Giordanengo, V., Doglio, A., Cagnon, L., Breittmayer, J. P., Peyron, J. F., and Lesimple, J. (1994) Virology 199, 265-274; Lefebvre, J. C., Giordanengo, V., Limouse, M., Doglio, A., Cucchiarini, M., Monpoux, F., Mariani, R., and Peyron, J. F. (1994) J. Exp. Med. 180, 1609-1617). Searching to clarify mechanism(s) of hyposialylation, we observed two sulfated secreted glycoproteins (molecular mass approximately 47 and approximately 40 kDa) (P47 and P40), which were differentially sulfated and/or differentially secreted in the culture supernatants of CEMLAI/NP cells when compared with parental CEM cells. A hybridoma clone (7H1) resulting from the fusion between CEMLAI/NP and human embryonic fibroblasts MRC5 cells produced very large amounts of P47 that was purified using Jacalin lectin (specific for O-glycans) and microsequenced. Cloning of P47 was achieved using a CEMLAI/NP cDNA library screened with a degenerate oligonucleotide probe based on its NH2-terminal amino acid sequence. A single open reading frame encoding...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1990·Annual Review of Immunology·D H Conrad
Jan 1, 1989·Methods in Enzymology·H Kaku, I J Goldstein
May 1, 1989·Journal of Cellular Biochemistry·S R Hull, K L Carraway
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Oct 1, 1988·The Journal of Experimental Medicine·R L BarkerL R Pease
Nov 26, 1986·Biochemical and Biophysical Research Communications·G HortinA W Strauss
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 1, 1995·European Journal of Immunology·V GiordanengoJ C Lefebvre
Nov 1, 1994·The Journal of Experimental Medicine·J C LefebvreJ F Peyron
Mar 1, 1994·Virology·J C LefebvreJ Lesimple
Oct 1, 1995·Current Opinion in Cell Biology·K M Yamada, S Miyamoto
Feb 1, 1997·European Journal of Biochemistry·H J Gabius
Jul 4, 1997·The Journal of Biological Chemistry·A YoshidaM Takeuchi
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·A HiraokaS Shimizu
Aug 29, 1997·The Journal of Biological Chemistry·J R BundgaardJ F Rehfeld

❮ Previous
Next ❯

Citations

Jan 24, 2002·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·C PerrinV Giordanengo
Dec 13, 2005·The FEBS Journal·Alex N Zelensky, Jill E Gready
Mar 18, 2000·The Journal of Biological Chemistry·C SassettiS D Rosen
Jun 30, 2018·International Journal of Molecular Medicine·Xiaoyan ZhaoHaibing Wu
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Scott M DrouinRick A Wetsel
Nov 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Farrah KheradmandDavid B Corry

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.