Molecular cloning of a novel 120-kDa TBP-interacting protein

Biochemical and Biophysical Research Communications
S YogosawaT A Tamura

Abstract

TATA-binding protein (TBP) is a central component for transcriptional regulation and is a target for various transcription regulators. Using histidine-tagged TBP as a ligand for affinity-purification of proteins bound to TBP, we purified a 120-kD protein, termed TBP-interacting protein 120 (TIP120), from rat liver nuclear extracts. The entire cDNA sequence of TIP120 contained an open reading frame encoding a novel polypeptide of 1230 amino acids. The recombinant TIP120 interacted directly with TBP under a physiological condition in vitro. Immunoprecipitation analysis indicated that TIP120 was associated with TBP in nuclear extracts. Interestingly, the N-terminal region of TIP120 exhibited sequence similarity to that of Drosophila TAF80, which was shown to bind directly to TBP. This novel TBP-binding protein is considered to participate in transcription regulation through the interaction with TBP.

Citations

Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K KayukawaT Tamura
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Ning Wei, Xing Wang Deng
Apr 10, 2003·Biochemical and Biophysical Research Communications·Kiyotaka OshikawaKeiichi I Nakayama
Oct 28, 2010·Molecular Plant·Xiao-Fei WangPing Wu
Sep 12, 1997·The Journal of Biological Chemistry·Y MakinoT Tamura
Mar 1, 2003·The Journal of Biological Chemistry·Kyoeng-Woo MinJong-Bok Yoon
Apr 15, 2003·The Journal of Biological Chemistry·Jianxin YouCecile M Pickart
Jan 7, 2004·The Journal of Biological Chemistry·Carin G M ZwartjesH T Marc Timmers
Sep 1, 2006·Development·María Magdalena Alonso-PeralJosé Luis Micol
Sep 5, 2002·Biochemical and Biophysical Research Communications·Tsutomu AokiTaka-aki Tamura
Jun 24, 2004·Seminars in Cell & Developmental Biology·Geraint Parry, Mark Estelle

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