Molecular cloning of a novel human gene encoding a 63-kDa protein and its sublocalization within the 11q13 locus

Genomics
B PerelmanD Canaani

Abstract

A human cDNA previously isolated by virtue of its ability to complement partially the ultraviolet sensitivity of a xeroderma pigmentosum cell line was further characterized. The transcription unit is expressed as a single 4.0-kb mRNA that encodes a novel 63-kDa cytoplasmic protein, possibly initiating from an internal AUG codon. The gene encoding this protein, named UVRAG, has been extremely well conserved during evolution, implying an important role for this gene product in cell metabolism. The transcribed mRNA is constitutively expressed in a wide variety of human tissues. The protein encoded by this gene is predicted to contain a coiled-coil structure and is likely to be metabolically unstable based on the occurrence of a strong PEST domain. UVRAG was assigned to human chromosome 11 by Southern hybridization to a somatic cell hybrid panel. Fluorescence in situ hybridization coupled with PCR analysis of human/rodent somatic cell hybrids containing segments of human chromosome 11 has localized this gene to a subregion of 11q13 in between the D11S916 and the D11S906 loci. Importantly, this region has been shown to be amplified in a variety of human malignancies, including breast cancer.

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Citations

Jun 27, 2006·Nature Cell Biology·Chengyu LiangJae U Jung
Oct 31, 2008·Nature Reviews. Molecular Cell Biology·Guido Kroemer, Beth Levine
Apr 8, 2009·Molecular Neurodegeneration·Philipp A Jaeger, Tony Wyss-Coray
Apr 14, 2009·Biological Chemistry·Anne Spang
Nov 5, 2013·Autoimmunity Reviews·Elena GianchecchiAlessandra Fierabracci
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Feb 19, 2010·Experimental Dermatology·Taek-Jo JeongMu-Hyoung Lee
Jul 7, 2011·Developmental Biology·Gina LeeJongkyeong Chung
Dec 10, 2016·Photochemistry and Photobiology·Ashley Sample, Yu-Ying He
Jan 21, 2017·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Vincent Hung-Shu ChangHui-Ju Ch'ang
Nov 8, 2017·International Journal of Molecular Sciences·Luciana R GomesGiovana S Leandro
Nov 7, 2018·Journal of Lipid Research·Yohei OhashiRoger L Williams
Jan 25, 2008·The Biochemical Journal·Jonathan M Backer

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