Molecular cloning of a novel variant of the pituitary adenylate cyclase-activating polypeptide (PACAP) receptor that stimulates calcium influx by activation of L-type calcium channels.

The Journal of Biological Chemistry
T K ChatterjeeR A Fisher

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a novel neuropeptide that produces its biological effects by interacting with G protein-coupled receptors. Molecular cloning of the PACAP receptor revealed the existence of five splice variant receptor forms differing in the third intracellular loop region, with four variants activating both adenylyl cyclase and phosphoinositide phospholipase C and one variant activating only adenylyl cyclase (Spengler, D., Waeber, C., Pantaloni, C., Holsboer, F., Bockaert, J., Seeburg, P. H., and Journot, L. (1993) Nature 365, 170-175). Here, we report cloning of a novel PACAP receptor variant, designated PACAPR TM4 (transmembrane domain IV), that differs from the previously cloned short form of the PACAP receptor (PACAPR) primarily by discrete sequences located in transmembrane domains II and IV. Reverse transcriptase-polymerase chain reaction and primer extension analyses demonstrated tissue-specific differential expression of mRNAs encoding PACAPR TM4 and splice variant forms of the PACAP receptor. PACAPR TM4 and PACAPR possess identical intracellular domains, implicated as primary determinants of G protein recognition by rhodopsin-like receptors. However, unlike the PACAPR, PACA...Continue Reading

References

Jan 1, 1992·Annual Review of Pharmacology and Toxicology·J OstrowskiR J Lefkowitz
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Thorens
Oct 16, 1989·Biochemical and Biophysical Research Communications·A MiyataD H Coy
Jan 13, 1995·The Journal of Biological Chemistry·P B WedegaertnerH R Bourne
Jan 1, 1994·Annual Review of Biochemistry·C D StraderR A Dixon
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·N InagakiS Seino
Oct 29, 1993·Biochimica Et Biophysica Acta·J Christophe
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J R Pisegna, S A Wank
Jan 1, 1993·The American Journal of Physiology·R V Sharma, R C Bhalla

❮ Previous
Next ❯

Citations

Jul 9, 2008·Journal of Molecular Neuroscience : MN·D ReglodiZ Helyes
Jul 3, 2008·Journal of Molecular Neuroscience : MN·Adriane D Sumner, Joseph F Margiotta
Aug 4, 1999·Trends in Pharmacological Sciences·T Dickinson, S M Fleetwood-Walker
Jul 3, 1999·Trends in Pharmacological Sciences·G J KilpatrickR M Eglen
Oct 26, 2002·Brain Research. Molecular Brain Research·Supaporn AjpruDavid Sugden
Jun 26, 2003·Brain Research. Molecular Brain Research·Ryusuke SuzukiSeiji Shioda
Mar 30, 1999·Brain Research. Molecular Brain Research·C OttoG Schütz
Oct 16, 1999·Brain Research. Molecular Brain Research·K PeetersF Vandesande
Nov 15, 2002·Trends in Endocrinology and Metabolism : TEM·Costanzo MorettiGaetano Frajese
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carmen MartinezRosa P Gomariz
Feb 3, 1999·Annals of the New York Academy of Sciences·S EmamiG Rosselin
Feb 3, 1999·Annals of the New York Academy of Sciences·J Christophe
Feb 3, 1999·Annals of the New York Academy of Sciences·M MuroiA Arimura
Feb 3, 1999·Annals of the New York Academy of Sciences·T YadaM Kikuchi
Apr 2, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Masanori Nakata, Toshihiko Yada
Dec 27, 2008·Pharmacology & Therapeutics·Louise Dickson, Keith Finlayson
Nov 7, 2008·Neuropeptides·K S MönkkönenC O'Callaghan
Jul 1, 2008·Respiratory Physiology & Neurobiology·Richard J A Wilson, Kevin J Cumming
Jun 15, 2007·Peptides·Maria Sörhede Winzell, Bo Ahrén
Dec 17, 2008·Annals of the New York Academy of Sciences·Bo Ahrén
Jun 6, 2014·Journal of Neurochemistry·Tommy SeabornDavid Vaudry
Feb 17, 2005·Molecular and Cellular Endocrinology·Erica A FradingerNancy M Sherwood

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.